Summary
The activity of alkaline phosphatase (AAP) in papillary capillaries of normal and psoriatic skin was characterized by enzyme inhibition studies. Quantitatively, there were pronounced differences between normal and psoriatic skin, i.e., increase of AAP, as determined by a grading system, and assimilation of the strength of AAP in venous and arterial side of the capillary loop in psoriasis.
Qualitatively, the inhibition studies with different acting inhibitors revealed no difference between AAP in normal and psoriatic skin or between initial, fully developed and healed psoriatic lesions as well as noninvolved skin of psoriatics. Thus, the physiologic AAP seems to be stimulated in psoriasis.
Generally, AAP of dermal capillaries is highly sensitive to cysteine inhibition.
Zusammenfassung
Die Aktivität der alkalischen Phosphatase (AAP) in papillären Kapillaren normaler und psoriatischer Haut wurde in Enzym-Inhibitionsversuchen charakterisiert. Quantitativ wurden erhebliche Unterschiede zwischen normaler und psoriatischer Haut festgestellt, insbesondere eine Zunahme der AAP sowie eine Angleichung zwischen venösem und arteriellem Schenkel der Kapillarschleife bei Psoriasis.
Qualitativ ergaben sich bei unseren Inhibitionsstudien mit verschieden wirksamen Inhibitoren keine Unterschiede zwischen der AAP bei normaler und psoriatischer Haut sowie zwischen der AAP bei initialen, voll entwickelten und abgeheilten Psoriasiseffloreszenzen oder nichtbefallener psoriatischer Haut. Somit scheint bei Psoriasis eine Stimulation der physiologischen AAP vorzuliegen.
Die AAP in dermalen Kapillaren ist durch eine hohe Empfindlichkeit gegenüber Cystein ausgezeichnet.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agus SG, Cox RP, Griffin MJ (1966) Inhibition of alkaline phosphatase by cysteine and its analogues. Biochim Biophys Acta 118:363–370
Borgers M (1973) The cytochemical application of new potent inhibitors of alkaline phosphatase. J Histochem Cytochem 21:812–824
Borgers M (1973) Levamisole and its analogue R 8231: new potent inhibitors of alkaline phosphatase. Electr Micr Cytochem 1973:51–54
Borgers M, Thone F (1975) The inhibition of alkaline phosphatase by L-P-bromotetramisole. Histochem 44:277–280
Braun-Falco O, Burg G (1970) Zur Histochemie der Capillaren bei Psoriasis vulgaris. Arch Klin Exp Dermatol 263:173–189
Braverman IM, Yen AB (1977) Ultrastructure of the capillary loops in the dermal papillae of psoriasis. J Invest Dermatol 68:53–60
Brunel C, Cathala G (1972) Imidazole: an inhibitor of L-phenylalanine — insensitive alkaline phosphatase of tissues other than intestine. Biochim Biophys Acta 268:415–421
Cormane RH (1962) Comparative enzyme-histochemical studies of the human skin. Dermatologica 124:359–366 (1962)
Cox RP, Griffin MJ (1967) Alkaline phosphatase. Biochim Biophys Acta 122:552–562
De Catelet LR, Volk JV, McCall CE, Cooper MR (1971) Studies on leukocyte phosphatase — Inhibition of leukocytic alkaline phosphatase by amino acids and its reversal by zinc. Clin Chem 17:210–213
Fishman WH (1974) Perspectives on Alkaline Phosphatase Isoenzymes. Am J Med 56:617–651
Fishman WH, Green S, Inglis NL (1962) Organ-specific behaviour exhibited by rat intestine and liver alkaline phosphatase. Biochim Biophys Acta 62:363–375
Fishman WH, Green S, Inglis NL (1963) L-phenylalanine: an organ specific, stereo specific inhibitor of human intestinal alkaline phosphatase. Nature 198:685–686
Ghosh NK, Fishman WH (1966) On the mechanism of inhibition of intestinal alkaline phosphatase by L-phenylalanine. J Biol Chem 241:2516–2522
Ghosh NK, Fishman WH (1968) L-phenylalanine inhibition of rat intestinal alkaline phosphatase: a homosteric phenomenon. Biochim Biophys Acta 126:700–706
Griffin MJ, Cox RP (1967) Studies on the mechanism of substrate induction and L-cyst(e)ine repression of alkaline phosphatase in mammalian cell cultures. J Cell Sci 2:545–555
Klingmüller G (1958) Die Darstellung alkalischer Phosphatase in Capillaren. Hautarzt 9:84–88
Kopf AW (1957) The distribution of alkaline phosphatase in normal and pathologic human skin. Arch Dermatol 76:1–37
Leder L (1967) Der Blutmonozyt. Springer, Berlin Heidelberg New York
Lin CW, Fishman WH (1972) L-Homoarginine: an organ-specific, uncompetetive inhibitor of human liver and bone alkaline phosphohydrolases. J Biol Chem 247:3082–3087
Lin CW, Sie HG, Fishman WH (1971) L-Tryptophane: a non allosteric organ-specific uncompetetive inhibitor of human placental alkaline phosphatase. Biochem J 124:509–516
Lustig V, Kellen JA (1971) Species, organ, and subcellular specifity of alkaline phosphatase as determined by amino acid inhibition studies. Comp Biochem Physiol 39:305–310
Moretti G (1968) In: Gans O, Steigleder GK (Hrsg) Handbuch der Haut- und Geschlechtskrankheiten, I/1. Springer, Berlin Heidelberg New York, pp 491–623
Ohlson JT, Wilson IB (1974) The inhibition of alkaline phosphatase by periodate and permanganate. Biochim Biophys Acta 350:48–53
Seifert HW, Klingmüller G (1972) Elektronenmikroskopische Struktur normaler Hautcapillaren und das Verhalten alkalischer Phosphatase. Arch Dermatol Res 242:97–110
Szodoray L, Sóvári E (1952) Untersuchungen der Gewebeenzyme der Haut bei Schuppenflechte. Acta Morphologica 18:110–119
Van Belle H (1972) Kinetics and inhibition of alkaline phosphatases from canine tissues. Biochim Biophys Acta 289:158–168
Wöltgens JHM (1977) Influence of various inhibitors on alkaline phosphatase in calcifying hamster molars employing p-nitrophenyl phosphate as substrate. J Dent Res 56:1255–1259
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Dr. W. Schneider on his 70th anniversary
Rights and permissions
About this article
Cite this article
Sterry, W., Steigleder, G.K. & Neumann, G. Characterization of activity of alkaline phosphatase (AAP) in capillary endothelium of normal and psoriatic skin. Arch Dermatol Res 267, 131–139 (1980). https://doi.org/10.1007/BF00569099
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00569099