Virus Genes

, Volume 5, Issue 3, pp 267–271 | Cite as

Nucleotide sequence of cDNA encoding the coat protein of beet yellows virus

  • Janne Brunstedt
  • Julie Moseley
  • Roger Hull
Article

Abstract

A cDNA clone of beet yellows viral RNA expressed the viral coat protein gene inE. coli. The sequence of the 2724 nucleotide insert revealed three open reading frames, the 3′ of which was shown to be the coat protein cistron. This cistron is expressed inE. coli, in spite of there being no obvious ribosome binding site upstream.

Key words

beet yellows virus viral coat protein K. coli expression closterovirus nucleotide sequence non-5′ open reading frame 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Janne Brunstedt
    • 1
  • Julie Moseley
    • 2
  • Roger Hull
    • 2
  1. 1.Danisco A/S, Biotechnology Research DivisionLangebrogadeDenmark
  2. 2.John Innes InstituteNorwichUK

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