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Chemistry of Natural Compounds

, Volume 5, Issue 6, pp 453–457 | Cite as

The separation of 1-dimethylaminonaphthalene-5-sulfonyl derivatives of peptides by two-dimensional electrophoresis in a thin layer of cellulose

  • G. S. Katrukha
  • Zh. P. Trifonova
  • A. B. Silaev
  • R. I. Li
Article
  • 24 Downloads

Conclusions

1. A new method for the separation of DNS-peptides has been proposed which is based on the use of two-dimensional electrophoresis in a thin layer of cellulose and which, in combination with photodocumentation, enables clear satisfactorily reducible “peptide maps” to be obtained with only 2–4×10−9 mole of protein (peptide) in a time not exceeding 3.5 hr.

2. The method described has been used to determine the number of DNS peptides in an incomplete acid hydrolysate of the DNS derivative of the antibiotic A-128-OP (acid).

Keywords

Cellulose Powder Sodium Bicarbonate Solution Aqueous Sodium Bicarbonate Pepsin Hydrolysate Aqueous Sodium Bicarbonate Solution 

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Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • G. S. Katrukha
    • 1
  • Zh. P. Trifonova
    • 1
  • A. B. Silaev
    • 1
  • R. I. Li
    • 1
  1. 1.Moscow State UniversityUSSR

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