The separation of 1-dimethylaminonaphthalene-5-sulfonyl derivatives of peptides by two-dimensional electrophoresis in a thin layer of cellulose
- 24 Downloads
1. A new method for the separation of DNS-peptides has been proposed which is based on the use of two-dimensional electrophoresis in a thin layer of cellulose and which, in combination with photodocumentation, enables clear satisfactorily reducible “peptide maps” to be obtained with only 2–4×10−9 mole of protein (peptide) in a time not exceeding 3.5 hr.
2. The method described has been used to determine the number of DNS peptides in an incomplete acid hydrolysate of the DNS derivative of the antibiotic A-128-OP (acid).
KeywordsCellulose Powder Sodium Bicarbonate Solution Aqueous Sodium Bicarbonate Pepsin Hydrolysate Aqueous Sodium Bicarbonate Solution
Unable to display preview. Download preview PDF.
- 3.J. Langner and Hoppe-Seylers, Z. Physiol. Chem.,347, 275, 1966.Google Scholar
- 5.A. B. Silaev, G. S. Katrukha, Zh. P. Trifonova, and I. Sinyavskaya, Antibiotiki,1, 13, 1968.Google Scholar
- 6.A. Pearse, Histochemistry: Theoretical and Applied [Russian translation], V. Portugalov, ed., Moscow, 720, 1962.Google Scholar
- 9.G. S. Katrukha, Zh. P. Trifonova, and A. B. Silaev, KhPS [Chemistry of Natural Compounds],2, 348, 1966.Google Scholar