Advertisement

Chemistry of Natural Compounds

, Volume 11, Issue 6, pp 768–771 | Cite as

Steroids LI. The transformation of a series of 20-oxosteroids by a culture of Bacillus megaterium VNIKhFI-1

  • L. A. Krasnova
  • L. B. Sokolova
  • N. A. Korzinkina
  • N. N. Suvorov
Article

Summary

The substrate specificity of an intact culture ofBacillus megaterium in relation to steroids of the pregnane series with a dihydroxyacetone side chain and without it, and also with steroids containing a 16α,17α-epoxy ring, has been studied.

It has been shown that a culture ofBac. megaterium performs the reduction of a 20-oxo to a 20α-hydroxy group only in the case of Δ4-3-oxo-steroids containing a 16α,17α-epoxy ring.

The necessity of a dihydroxyacetone side chain for the β-reduction of a 20-oxo group has been established.

Keywords

Manganese Dioxide Epoxy Ring Deoxycorticosterone Deuterochloroform Sodium Tetrahydroborate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    L. A. Krasmova, L. V. Sokolova, V. M. Ryzhkova, and N. N. Suvorov, Prikl. Biokhim. i Mikrobiol.,5, 260 (1969).Google Scholar
  2. 2.
    L. A. Krasnova, L. V. Sokolova, and N. N. Suvorov, Mikrobiol. Prom.,11, 24 (1973).Google Scholar
  3. 3.
    V. M. Chang and D. R. Idler, Canad. J. Biochem. Physiol.,39, 1277 (1961).CrossRefGoogle Scholar
  4. 4.
    L. M. Kogan, É. A. Elin, V. I. Mel'nikova, and I. V. Torgov, Khim. Prirodn. Soedin., 149 (1969).Google Scholar
  5. 5.
    R. Plaurde, O. M. El-Tayeb, and H. Hafez-Zedan, Appl. Microbiol.,23, 601 (1972).Google Scholar
  6. 6.
    F. Lindner, R. Junk, Y. Nesemann, and J. Schmidt-Thame, Z. Physiol. Chrm.,313, 117 (1958).CrossRefGoogle Scholar
  7. 7.
    L. M. Kogan, V. I. Ulezlo, K. G. Suvorov, and I. V. Torgov, Izv. Akad. Nauk SSSR., Otd. Khim. Nauk, No. 2, 328 (1963).Google Scholar
  8. 8.
    S. A. Szpilfogel, M. S. De Winter, and W. I. Alsche, Rec. Trav. Chim.,75, 402 (1956).CrossRefGoogle Scholar
  9. 9.
    J. E. Wilson and C. S. Vestling, Arch. Bioch. Biophys.,110, 401 (1965).CrossRefGoogle Scholar
  10. 10.
    Z. H. Sarett, J. Amer. Chem. Soc.,71, 1175 (1949).CrossRefGoogle Scholar
  11. 11.
    R. E. Burton, A. Zaffaroni, and E. H. Keutmann, J. Biol. Chem.,188, 763 (1951).PubMedGoogle Scholar
  12. 12.
    A. A. Akhrem and A. I. Kuznetsov, Thin-Layer Chromatography [in Russian], Moscow (1964).Google Scholar
  13. 13.
    P. Steyermark and W. Nawaczynski, Arch. Bioch. Biophys.,59, 1 (1955).CrossRefGoogle Scholar
  14. 14.
    N. A. Yudaev, Chemical Methods of Determining Steroid Hormones in Biological Liquids [in Russian], Moscow (1961).Google Scholar
  15. 15.
    E. Vizcher, Ch. Meystre, and A. Wettstein, Helv, Chim, Acta,38, 835 (1955).CrossRefGoogle Scholar
  16. 16.
    R. M. Dodson and C. G. Bergstrom, US. Patent No. 2951857; Chem. Abstr.55, P4591b (1960).Google Scholar

Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • L. A. Krasnova
  • L. B. Sokolova
  • N. A. Korzinkina
  • N. N. Suvorov

There are no affiliations available

Personalised recommendations