Chemistry of Natural Compounds

, Volume 16, Issue 4, pp 405–408 | Cite as

Isolation and purification of biopolymers by affinity chromatography. VI. Preparation and properties of an affinity adsorbent with a polysaccharide spacer for the purification of proteolytic enzymes

  • V. Kh. Mitina
  • R. I. Yakubovskaya
  • B. A. Klyashchitskii
  • F. M. Shemyakin


A new affinity sorbent has been synthesized — soybean trypsin inhibitor (STI)-amylopectin-hydrazidosuccinyl-Sepharose — and its properties have been studied in comparison with those of an analogous adsorbent without the spacer STI-Sepharose. The STI-amylopectin-hydrazidosuccinyl-Sepharose adsorbent has been used for the purification of trypsin from porcine pancreas and of callicrein from human blood plasma.


Soybean Trypsin Inhibitor Cyanogen Bromide Human Blood Plasma Affinity Adsorbent Porcine Pancreas 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. 1.
    B. A. Klyashchitskii, V. F. Pozdner, V. Kh. Mitina, A. I. Voskoboev, and I. P. Chernikevich, Bioorg. Khim.,6 (1980).Google Scholar
  2. 2.
    H. Ako, R. J. Foster, and C. A. Ryan, Biochemistry,13, 132 (1974).CrossRefGoogle Scholar
  3. 3.
    R. S. Temler and J. H. R. Kagi, Enzyme,22, 249 (1977).PubMedGoogle Scholar
  4. 4.
    S. F. Russo and D. J. Yadoff, Comp. Biochem. Physiol.,60, 453 (1978).CrossRefGoogle Scholar
  5. 5.
    H. Heber, R. Geiger, and N. H. Heimburger, Z. Physiol. Chem.,359, 659 (1978).Google Scholar
  6. 6.
    S. Sampaio, S.-C. Wong, and W. E. Shaw, Arch. Biochem. Biophys.,165, 133 (1974).CrossRefGoogle Scholar
  7. 7.
    J. Porath, R. Axen, and S. Ernback, Nature (London),215, 1491 (1967).CrossRefGoogle Scholar
  8. 8.
    I. Karube, Y. Ishimori, and S. Suzuki, Anal. Biochem.,86, 100 (1978).CrossRefGoogle Scholar
  9. 9.
    B. A. Klyashchitskii and V. Kh. Mitina, Zh. Obsh. Khim.,48, 913 (1978).Google Scholar
  10. 10.
    B. A. Klyashchitskii, G. E. Koroleva, V. Kh. Mitina, R. P. Alekhina, I. B. Zborovskaya, and A. B. Likhtenshtein, Bioorg. Khim.,5, 92 (1979).Google Scholar
  11. 11.
    A. Miyagawa and T. Okuyama, J. Biochem.,81, 1715 (1977).CrossRefGoogle Scholar
  12. 12.
    H. Tchesche, Angew. Chem.,13, 10 (1974).CrossRefGoogle Scholar
  13. 13.
    T. S. Paskhina, A. V. Krinskaya, and V. G. Zylova, Biokhimiya,40, 302 (1975).Google Scholar

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • V. Kh. Mitina
  • R. I. Yakubovskaya
  • B. A. Klyashchitskii
  • F. M. Shemyakin

There are no affiliations available

Personalised recommendations