Abstract
Two monosaccharides have been found by paper chromatography in an aqueous extract of the herbage of red clover (Trifolium pratense). One of them has been identified as glucose. After hydrolysis of a dry water-soluble extract by paper chromatography in the presence of markers, galactose, arabinose, xylose, and mannose were detected. By paper chromatography in the presence of markers the amino acids phenylalanine, leucine (isoleucine), methionine, aspartic acid, proline, alanine, and histidine have been identified. Phytin with decomp. p 276°C has been isolated and identified, giving, after hydrolysis, inositol with mp 225–226°C, and Ca2+, Mg2+, and PO 3−4 have been detected. The amount of ash in the herbage of clover is 8.4% and the amount insoluble in HC1 1.5%. The amounts of macroelements were determined by the flame photometry of a solution of the ash (mg−%): K+ 1620; Na+ 310; Ca2+ 1240; Mg2+ 1090.
Similar content being viewed by others
Literature cited
N. G. Kovaleva, in: Medicinal Plants [in Russian], Moscow (1971), p. 34.
A. F. Gamerman and P. I. Grom, Wild Medicinal Plants of the USSR [in Russian], Moscow (1976), p. 132.
J. Macku and J. Kreča, in: Atlas of Medicinal Plants [in Czech], Bratislava (1970), p. 186.
V. Florya, in: Medicinal Plants [in Russian], Kishinev (1976), p. 224.
E. V. Kucherov, G. K. Baikov, and I. B. Gufranov, in: Useful Plants of the Southern Urals [in Russian], Moscow (1976), p. 135.
A. L. Kazakov, V. I. Litvinenko, and A. S. Ammosov, Khim. Prir. Soedin., 432 (1973).
A. L. Kazakov, A. L. Shinkarenko, and É. T. Oganesyan, Izv. Sev. Kavkaz. Nauchn. Tsentra Vyssh. Shkoly. Ser. Tekhn. Nauki,4, 82 (1973).
A. L. Kazakov and T. P. Leont'eva, Medicinal Agents [in Russian], (G. N. Dorofeenko, ed.), Rostov (1979), p. 145.
A. L. Kazakov and D. M. Eliseevich, Khim. Prir. Soedin., 258 (1976).
A. P. Kreshkov, Principles of Analytical Chemistry [in Russian], Vol. 1, Moscow (1976).
J. Scherer, Ann. Chem. Pharm.,81, 375 (1952).
S. I. Dzyumyrko and A. L. Shinkarenko, Khim. Prir. Soedin., 384 (1972).
Methods for the Quantitative Paper Chromatography of Sugars, Organic Acids, and Amino Acids in Plants [in Russian], Moscow-Leningrad (1962).
Yu. A. Zhdanov, “The chromatography of the sugars,” in: Practical Handbook on Carbohydrate Chemistry [in Russian], Moscow (1973), p. 185.
I. M. Roze and T. G. Savel'eva, Khim. Drev.,7, 119 (1971).
Additional information
Pyatigorsk Pharmaceutical Institute. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 483–485, July–August, 1980.
Rights and permissions
About this article
Cite this article
Kazakov, A.L., Samokish, I.I. Carbohydrates, amino acids, phytin, and macroelements of the herbage ofTrifolium pratense . Chem Nat Compd 16, 343–345 (1980). https://doi.org/10.1007/BF00568363
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00568363