Chemistry of Natural Compounds

, Volume 16, Issue 4, pp 338–342 | Cite as

A study of the products of the hydrolysis of the xylan ofMelilotus albus by endo-1 4-β-xylanase

  • M. S. Dudkin
  • N. A. Rodionova
  • I. S. Kazanskaya
  • I. V. Gorbacheva
  • E. I. Kozarez
  • N. A. Denisyuk


The enzymatic hydrolysis of a 4-0-methylglucuronoxylan of white sweetclover (Melilotus albus) by a highly purified homogeneous endo-1, 4-β-xylanase fromAspergillus niger 14 has shown that the enzyme hydrolyzes 97% of the polysaccharide in 72 h. Acidic and neutral oligosaccharides were found in the hydrolysate after the action of the enzyme. An investigation of the hexauronic acid isolated has shown that the glucuronic acid is attached to the nonreducing end of the xylooligosaccharide, which demonstrates the specific action of the enzyme on the polysaccharide.


Xylose Oligosaccharide Glucuronic Acid Xylobiose Dimethyl Sulfate 


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Literature cited

  1. 1.
    N. A. Rodionova, R. V. Feniksova, and I. V. Itomlenskite, Khim. Drev.,8, 127 (1971).Google Scholar
  2. 2.
    I. V. Gorbacheva and N. A. Rodionova, Biochem. Biophys. Acta,484, 79 (1977).PubMedGoogle Scholar
  3. 3.
    N. A. Rodionova, I. V. Gorbacheva, and V. A. Buivid, Biokhimiya,42, No. 4, 659 (1977).Google Scholar
  4. 4.
    R. F. H. Dekker and G. N. Richards, Adv. Carbohydr. Chem.,32, 346 (1976).Google Scholar
  5. 5.
    T. E. Timell, Svensk. Papperstidn.,65, 435 (1962).Google Scholar
  6. 6.
    M. Sinner, Mitt. Bundesforschunganstalt Forst, Holz-Wirtsch.,93, 257 (1973).Google Scholar
  7. 7.
    M. S. Dudkin and N. G. Shkantova, Abstracts of Lectures at an All-Union Conference on Processing Products of the Timber and Agricultural Industries [in Russian], Riga (1973), p. 24.Google Scholar
  8. 8.
    G. H. Roe, J. Biol. Chem.,212, 335 (1955).PubMedGoogle Scholar
  9. 9.
    E. L. Hirst and E. Percival, in: Methods of Carbohydrate Chemistry (R. L. Whistler, ed.), Vol. 5, Academic Press, New York (1965), pp. 289–290.Google Scholar
  10. 10.
    C. J. Giang and R. H. Marchessault, J. Polymer Sci.,39, 269 (1959).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • M. S. Dudkin
  • N. A. Rodionova
  • I. S. Kazanskaya
  • I. V. Gorbacheva
  • E. I. Kozarez
  • N. A. Denisyuk

There are no affiliations available

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