Skip to main content
Log in

Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations

  • Short Communications
  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

Cis-dichloro-diammine platinum (II) provides a strong contrast to electron microscopic preparations by reacting with nucleic acids and proteins. This staining technique is easy and applicable to ultrathin sections embedded in Epon; various staining conditions have been tested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Aggarwal, S.K., Wagner, R.W., McAllister, P.K., Rosenberg, B.: Cell-surface-associated nucleic acid in tumorigenic cells make visible with platinum-pyrimidine complexes by electron microscopy. Proc. nat. Acad. Sci. (Wash.)12, 928–932 (1973)

    Google Scholar 

  • Davidson, J.P., Faber, P.J., Fischer, R.G., Mansy, S., Peresie, H.J., Rosenberg, B., Van Camp, L.: Platinum-pyrimidine blues and related complexes. A new class of antitumor agents. Cancer Chemother. Rep.59, 287–300 (1975)

    Google Scholar 

  • Millard, M.M., Macquet, J.P., Theophanides, T.: X-ray photoelectron spectroscopy of DNA-Pt complexes. Evidence of O6(Gua). N7(Gua) chelation of DNA with cis-dichloro-diammine platinum (II). Biochim. Biophys. Acta (Amst.)402, 166–170 (1975)

    Google Scholar 

  • Robins, A.B.: Interactions with Biomolecules. In: Platinum coordination complexes in cancer chemotherapy (T.A. Connors and J.J. Roberts, ed.), pp. 63–78. Berlin-Heidelberg-New York: Springer 1974

    Google Scholar 

  • Thompson, A.J.: The interactions of platinum compounds with biological molecules. In: Platinum coordination complexes in cancer chemotherapy (T.A. Connors and J.J. Roberts, ed.), pp. 38–62. Berlin-Heidelberg-New York: Springer 1974

    Google Scholar 

  • Wagner, R.W., Rosenberg, B., Aggarwal, S.K.: Platinum compounds as potential electron microscope stains: compounds containing uracil and thymine. Fed. Proc.33, 1385 (1974)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heinen, E. Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations. Histochemistry 51, 257–260 (1977). https://doi.org/10.1007/BF00567230

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00567230

Keywords

Navigation