Summary
A new use of the histochemical method for intestinal lactase activity is described. Peroral intestinal biopsies from newborn light-treated infants with diarrhoea were investigated for brush-border lactase. The lactase activity found in these infants by the histochemical method correlated well with the infants ability to hydrolyze lactose judged by lactose tolerance test.
References
Bakken, A.F.: Coeliaki hos barn. T. norske Lægeforen.94, 368–370 (1974)
Cremer, R.J., Perryman, P.W., Richards, D.H.: Influence of light on the hyperbilirubinemia of infants. Lancet1958I, 1094–1096
Lojda, Z., Kraml, J.: Indigogenic methods for glycosidases. III. An improved method with 4-Cl-5-Br-3-indolyl-β-D-fucoside and its application in studies of enzymes in the intestine, kidney and other tissues. Histochemie25, 195–207 (1971)
Lucey, J.E.: Phototherapy of jaundice. In: Bilirubin metabolism in the newborn, ed. Bergsma, D., Birth defects: original article series VI, pp. 63–70. The National Foundation. Williams and Wiklins Co. Baltimore: 1970
Lund, H.T., Jacobsen, J.: Influence of phototherapy on unconjugated bilirubin in duodenal bile of newborn infants with hyperbilirubinemia. Acta paediat. scand.61, 693–696 (1972)
Zetterström, R., Ernster, L.: Bilirubin, an uncoupler of oxidative phosphorylation in isolated mitochondria. Nature (Lond.)178, 1335–1337 (1956)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bakken, A.F. The application of the “Lojda” method for intestinal lactase in intestinal biopsies from jaundiced newborn infants. Histochemistry 51, 253–255 (1977). https://doi.org/10.1007/BF00567229
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00567229