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Demonstration of acetylcholinesterase by semipermeable membrane technique: Estimation of soluble and fixation-labile portions in different regions of the central nervous system

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Summary

A histochemical method for the demonstration of acetylcholinesterase using semipermeable membranes is described. This technique prevents any loss of enzyme activity caused by dissolution and/or fixation. The soluble and fixation-labile portions of acetylcholinesterase were estimated in several regions of the central nervous system of the rat and differences were found. The method improves the accuracy of the histochemical demonstration of the acetylcholinesterase on the light microscopical level.

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Authors and Affiliations

  1. Division of Biological Sciences, Karl-Marx-University Leipzig, Talstraße 33, DDR-701, Leipzig, German Democratic Republic

    Gerald Hüther & Hans Luppa

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  1. Gerald Hüther
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  2. Hans Luppa
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The research reported in this paper was supported by the “Ministerium für Wissenschaft und Technik der DDR”

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Hüther, G., Luppa, H. Demonstration of acetylcholinesterase by semipermeable membrane technique: Estimation of soluble and fixation-labile portions in different regions of the central nervous system. Histochemistry 51, 245–251 (1977). https://doi.org/10.1007/BF00567228

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  • DOI: https://doi.org/10.1007/BF00567228

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