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Histochemistry

, Volume 51, Issue 2–3, pp 141–152 | Cite as

Reduction and azo coupling of quinones

A histochemical study of human cutaneous melanin and adrenochrome
  • Ralph D. Lillie
  • Patricia T. Donaldson
  • Linda L. Vacca
  • Philip P. Pizzolato
  • Shitalkumar K. Jirge
Article

Summary

Cutaneous melanin in formol fixed skin and adrenochrome in dichromate fixed monkey adrenal after adequate bisulfite or dithonite reduction were found to give definite azo coupling reactions. Weaker reactions were obtained on unreduced material, and these disappeared on ferric chloride oxidation. Both cutaneous melanin and adrenochrome appear to exist in a quinhydrone status. Prolongation of dichromate treatment weakens or abolishes azo coupling capacity of adrenochrome. The findings support the concept of quinonization and reduction to prevent and restore azo coupling of enterochromaffin cells and noradrenaline islets of the adrenal.

The most effective diazos for melanin werep-nitrodiazobenzene, fast black K and the diazosulfanilic acid, pH 1 pyronin B procedure, for adrenochrome. Diazosafranin and 2-chloro-4-nitrodiazobenzene were also useful. Blue and violet coupling products from toluidine blue and methylene violet RR fail to yield sufficient contrast to be convincing.

Keywords

Bisulfite Toluidine Blue Ferric Chloride Dichromate Methylene Violet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Ralph D. Lillie
    • 1
  • Patricia T. Donaldson
    • 1
  • Linda L. Vacca
    • 1
  • Philip P. Pizzolato
    • 1
  • Shitalkumar K. Jirge
    • 1
  1. 1.Department of PathologyLouisiana State University Medical CenterNew OrleansUSA

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