Histochemistry

, Volume 51, Issue 2–3, pp 103–111 | Cite as

Banding isolated metaphase chromosomes by a sequential fluorescent G/Q technique

  • Joannie B. Krajca
  • Wayne Wray
Article

Summary

G/Q-banding is a new, rapid, fluorescent technique for banding isolated chromosomes that incorporates characteristics of both G- and Q-banding. G-bands, while easily characterized, are often inconsistent when using isolated chromosomes, and Q-bands, while reliable, fade rapidly under UV exposure, making prolonged observation and photography difficult. G/Q-banding combines these techniques to sequentially utilize quinacrine staining over Giemsa banding to produce slow-fading fluorescent G/Q-bands. The background fluorescence in G/Q preparations fades quickly under continued UV exposure, while the chromosomes remain brightly banded and can be observed and photographed for at least five minutes. G/Q-banding was extended to whole cell chromosome spreads and produced results identical to those obtained with isolated chromosomes. Whole cell karyotypes indicate that G/Q-bands generally correspond to Q-bands. Advantages of G/Q-banding as a unique and universal technique over current double-staining procedures are discussed.

Keywords

Public Health Metaphase Chromosome Background Fluorescence Quinacrine Fluorescent Technique 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chen, T.R.: A simple method to sequentially reveal Q- and C-bands on the same metaphase chromosomes. Chromosoma (Berl.)47, 147–156 (1974)Google Scholar
  2. Dev, V.G., Grewal, M.S., Miller, D.A., Kouri, R.E., Hutton, J.J., Miller, O.J.: The quinacrine fluorescence karyotype ofMus musculus and demonstration of strain differences in secondary constrictions. Cytogenet. Cell Genet.10, 436–451 (1971)Google Scholar
  3. Gagné, R., Tanguay, R., Laberge, C.: Differential staining patterns of heterochromatin in man. Nature (Lond.) New Biol.232, 29–30 (1971)Google Scholar
  4. Hsu, T.C.: Longitudinal differentiation of chromosomes. Ann. Rev. Genet.7, 153–176 (1973)Google Scholar
  5. Lubs, H.A., McKenzie, W.H., Patil, S.R., Merrick, S.: New staining methods for chromosomes. In: Methods in cell biology, Vol. 6 (D.M. Prescott, ed.), pp. 345–380. New York: Academic Press 1973Google Scholar
  6. Schnedl, W.: Banding patterns in human chromosomes visualized by Giemsa staining after various pretreatments. In: Methods in human cytogenetics (H.G. Schwarzacher, U. Wolf, E. Passarge, eds.), pp. 95–117. Berlin-Heidelberg-New York: Springer 1974Google Scholar
  7. Schwarzacher, H.G.: Fluorescence microscopy of chromosomes and interphase nuclei. In: Methods in human cytogenetics (H.G. Schwarzacher, U. Wolf, E. Passarge, eds.), pp. 83–94. Berlin-Heidelberg-New York: Springer 1974Google Scholar
  8. Stefos, K., Wray, W.: Cytologia, in press (1976)Google Scholar
  9. Wray, W.: Isolation of metaphase chromosomes, mitotic apparatus, and nuclei. In: Methods in cell biology, Vol. 6 (D.M. Prescott, ed.), pp. 283–306. New York: Academic Press 1973Google Scholar
  10. Wray, W.: Isopycnic centrifugation of mammalian metaphase chromosomes in metrizamide. FEBS Letters62, 202–207 (1976)Google Scholar
  11. Wray, W., Stefos, K.: Isopycnic centrifugation and absolute identification of isolated metaphase chromosomes. J. Cell Biol.63, 380a (1974)Google Scholar
  12. Wray, W., Stubblefield, E.: A new method for the rapid isolation of chromosomes, mitotic apparatus, or nuclei from mammalian fibroblasts at near neutral pH. Exp. Cell Res.59, 469–478 (1970)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Joannie B. Krajca
    • 1
  • Wayne Wray
    • 2
  1. 1.University of Texas Graduate School of Biomedical SciencesHoustonUSA
  2. 2.Department of Cell BiologyBaylor College of MedicineHoustonUSA

Personalised recommendations