, Volume 51, Issue 2–3, pp 103–111 | Cite as

Banding isolated metaphase chromosomes by a sequential fluorescent G/Q technique

  • Joannie B. Krajca
  • Wayne Wray


G/Q-banding is a new, rapid, fluorescent technique for banding isolated chromosomes that incorporates characteristics of both G- and Q-banding. G-bands, while easily characterized, are often inconsistent when using isolated chromosomes, and Q-bands, while reliable, fade rapidly under UV exposure, making prolonged observation and photography difficult. G/Q-banding combines these techniques to sequentially utilize quinacrine staining over Giemsa banding to produce slow-fading fluorescent G/Q-bands. The background fluorescence in G/Q preparations fades quickly under continued UV exposure, while the chromosomes remain brightly banded and can be observed and photographed for at least five minutes. G/Q-banding was extended to whole cell chromosome spreads and produced results identical to those obtained with isolated chromosomes. Whole cell karyotypes indicate that G/Q-bands generally correspond to Q-bands. Advantages of G/Q-banding as a unique and universal technique over current double-staining procedures are discussed.


Public Health Metaphase Chromosome Background Fluorescence Quinacrine Fluorescent Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Joannie B. Krajca
    • 1
  • Wayne Wray
    • 2
  1. 1.University of Texas Graduate School of Biomedical SciencesHoustonUSA
  2. 2.Department of Cell BiologyBaylor College of MedicineHoustonUSA

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