Chemistry of Natural Compounds

, Volume 15, Issue 5, pp 521–523 | Cite as

Spectrophotometric determination of a bacterial lipopolysaccharide fromEscherichia coli 675

  • L. E. Shchedrina
  • L. I. Brutko


A method has been developed for the quantitative spectrophotometric determination of the lipopolysaccharides ofE. coli 675. The maximum absorption of the lipopoly-saccharides fromE. coli 675 (259 nm) coincides with the maximum absorption (259 nm) of a number of polysaccharides from other Gram-negative bacteria, which can be used to determine lipopolysaccharides in various media.


Spectrophotometric Determination Galacturonic Acid Chromic Acid Bacterial Origin Neutral Monosaccharide 


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Literature cited

  1. 1.
    J. Janda and E. Work, FEBS Letters,16, 340 (1971).CrossRefGoogle Scholar
  2. 2.
    N. A. Karamian, J. Pharm. Sci.,66, 723 (1977).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • L. E. Shchedrina
  • L. I. Brutko

There are no affiliations available

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