Chemistry of Natural Compounds

, Volume 13, Issue 4, pp 471–473 | Cite as

Isolation of an intracellular lipase from the heat-tolerant fungus Rhizopus microsporus UzLT-1 and its properties

  • K. D. Davranov
  • Zh. Kh. Duyarov
Article

Summary

1. A method of isolating and purifying an intracellular lipase of the fungusRhizopus microsporus, UzLT-1, has been described.

2. Some properties of the purified enzyme have been studied and it has been shown that the intracellular lipase differs from the extracellular lipase in its pH optimum and electrophoretic mobility.

3. The presence of sodium chloride (0.06 M) in the reaction mixture raises the heat stability of the purified lipase.

Keywords

Lipase Lipase Activity Extracellular Lipase Disk Electrophoresis Lipase Preparation 

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Literature cited

  1. 1.
    K. Davranov and M. Rizaeva, Khim. Prirodn. Soedin., 279 (1975).Google Scholar
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    K. Davranov, M. Z. Zakirov, and M. I. Rizaeva, Khim. Prirodn. Soedin., 636 (1976).Google Scholar
  3. 3.
    M. Rizaeva and K. D. Davranov, Prikl. Biokhim. i Mikrobiol, 383 (1976).Google Scholar
  4. 4.
    H. Maurer, Disk-Elektrophorese, Theorie und Praxis, Walter de Gruyter, Berlin (1968).Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • K. D. Davranov
  • Zh. Kh. Duyarov

There are no affiliations available

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