Chemistry of Natural Compounds

, Volume 13, Issue 6, pp 719–722 | Cite as

Isolation of ribosomes from cotton seeds

  • A. M. Érmatov
  • R. N. Nuriddinov


1. It has been established that the method of precipitation with ethanol in the presence of an increased concentration of Mg2+ can be used for the rapid isolation of the ribosome fraction from plant material.

2. The optimum conditions for the complete precipitation of the ribosome fraction from a homogenate of cotton seeds have been selected.

3. By treating an extract of the seeds with 0.5 M KCl it was possible to reduce the amount of tRNA in a preparation of the total rRNA.


Razor Blade Cotton Seed Ammonium Persulfate Leucite Rapid Isolation 


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Literature cited

  1. 1.
    M. G. Hamilton and M. L. Peterman, J. Biol. Chem.,234, 1141 (1959).Google Scholar
  2. 2.
    B. A. Larkins and E. Davies, Plant Physiol.,52, 655 (1973).CrossRefGoogle Scholar
  3. 3.
    Ts. V. Grancharova and T. A. Getova, Phytochemistry,15, 1431 (1976).CrossRefGoogle Scholar
  4. 4.
    H. Diesperger, C. R. Müller, and H. Sandermann, FEBS Lett.,43, 155 (1974).CrossRefGoogle Scholar
  5. 5.
    M. Koka and T. Makamoto, Biochem. Biophys. Acta,262, 381 (1972).PubMedGoogle Scholar
  6. 6.
    M. S. Kaulenas, Anal. Biochem.,41, 126 (1971).CrossRefGoogle Scholar
  7. 7.
    N. G. Avadhani and D. E. Buetow, Biochem. Biophys. Res. Commun.,50, 443 (1973).CrossRefGoogle Scholar
  8. 8.
    A. S. Spirin, Biokhimiya,23 656 (1958).Google Scholar
  9. 9.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem.,193, 265 (1951).Google Scholar
  10. 10.
    P. J. Payne and T. A. Dyer, Biochem. Biophys. Acta,228, 167 (1971).PubMedGoogle Scholar
  11. 11.
    H. Maurer, Disk-Elektrophorese, Walter de Gruyter, Berlin (1968).Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • A. M. Érmatov
  • R. N. Nuriddinov

There are no affiliations available

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