Chemistry of Natural Compounds

, Volume 14, Issue 4, pp 415–420 | Cite as

Isolation of the cytoplasmatic 5S ribosomal RNA from cotton seeds by preparative electrophoresis

  • A. M. Érmatov
  • R. N. Nuriddinov


1. The complete separation of low-molecular-weight 5S rRNA and tRNA from fractions of high-molecular-weight rRNAs (28S + 18S) in a preparation of the total rRNA from cotton seeds has been achieved.

2. The optimum conditions have been selected for the preparative electrophoresis and isolation of homogeneous preparations of 5S rRNA and total tRNA.


Cotton Seed Saturated NaCI Solution Anchor Group Disodium EDTA Rubber Gasket 


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Literature cited

  1. 1.
    A. M. Érmatov and P. N. Nuriddinov, Khim. Prirodn. Soedin., 852 (1977).Google Scholar
  2. 2.
    S. H. Wilson and R. V. Quincey, J. Biol. Chem.,244, No. 5, 1092 (1969).PubMedGoogle Scholar
  3. 3.
    M. A. Q. Siddiqui, J. Sci. Ind. Res. (India),32, No. 1, 34 (1973).Google Scholar
  4. 4.
    R. Villems and A. Lind, Uch. Zap. Tartuskogo Univ., No. 285, 82 (1971).Google Scholar
  5. 5.
    P. J. Payne and T. A. Dyer, Biochem. J.,124, No. 1, 83 (1971).CrossRefGoogle Scholar
  6. 6.
    J. D. Mandell and A. D. Hershey, Anal. Biochem.,1, 66 (1960).CrossRefGoogle Scholar
  7. 7.
    U. E. Loening, Biochem. J.,113, 131 (1969).CrossRefGoogle Scholar
  8. 8.
    H. Weber, M. A. Billeter, S. Kahane, C. Weismann, J. Hindley, and A. Porter, Nature, New Biology,237, 166 (1972).CrossRefGoogle Scholar
  9. 9.
    V. P. Kalinovskii, T. A. Goryukhina, S. D. Ivanova, and I. F. Seits, Lab. Delo,5, 280 (1974).PubMedGoogle Scholar
  10. 10.
    D. Racusen and M. Foote, Can. J. Botany,44, 1633 (1966).CrossRefGoogle Scholar
  11. 11.
    H. Maurer, Disk-Elektrophorese, Theorie und Praxis, Walter de Druyter, Berlin (1968).Google Scholar
  12. 12.
    L. S. Mil'man, Biokhimiya,25, No. 5, 796 (1960).Google Scholar
  13. 13.
    P. J. Payne and T. A. Dyer, Biochim. Biophys. Acta,228, No. 167 (1971).Google Scholar

Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • A. M. Érmatov
  • R. N. Nuriddinov

There are no affiliations available

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