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Chemistry of Natural Compounds

, Volume 15, Issue 4, pp 476–480 | Cite as

Action of trypsin on a nucleoprotein from sturgeon gonads

  • A. N. Bulanova
  • E. P. Yulikova
  • I. A. Kashirin
Article
  • 25 Downloads

Abstract

A nucleoprotein has been isolated from the gonads of the Caspian sturgeon and its composition has been determined. It has been shown that it contains 55% of DNA, 2% of RNA, 36% of protamines, and about 7% of nonprotamine proteins of nonbasic nature. The nucleoprotein has been hydrolyzed with trypsin, and the amino acid compositions of some hydrolysis products have been studied. On the basis of the results obtained, the hypothesis has been put forward of a possible linkage of the DNA with the basic proteins. It has been shown that protamines react with the DNA through the basic amino acid residues located at various regions of their molecules.

Keywords

Amino Acid Composition Protamine Acidic Amino Acid Benzenesulfonyl Basic Amino Acid Residue 

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Literature cited

  1. 1.
    J. Kato and K. Iwai, J. Biochem.,81, 621 (1977).CrossRefGoogle Scholar
  2. 2.
    A. N. Bulanova, E. P. Yulikova, L. A. Baratova, L. P. Belyanova, and A. B. Silaev, Soviet-Americian Symposium the Physics and Chemistry of Proteins, August 4–8, Abstracts of Lectures [in Russian], Riga (1976), p. 63.Google Scholar
  3. 3.
    E. P. Yulikova, L. K. Evseenko, L. A. Baratova, L. P. Belyanova, V. K. Rybin, and A. B. Silaev, Bioorg. Khim.,2, 1613 (1976).Google Scholar
  4. 4.
    V. A. Kadykov, G. Ya. Kolomiitseva, T. A. Manamsh'yan, E. P. Senchenkov, and M. A. Chepyzhova, Mol. Biol.,7, 232 (1973).Google Scholar
  5. 5.
    A. S. Spirin, Biokhimiya,23, 656 (1958).Google Scholar
  6. 6.
    J. Davidson and R. Smellie, Biochem. J.,52, 594 (1952).CrossRefGoogle Scholar
  7. 7.
    N. Sugano and S. Okada, Biochim. Biophys. Acta,383, 78 (1975).CrossRefGoogle Scholar
  8. 8.
    S. Sakaguchi, J. Biochem.,37, 231 (1950).CrossRefGoogle Scholar
  9. 9.
    B. G. Belen'kii, É. S. Gankina, S. A. Pryanishnikova, and D. T. Érastov, Mol. Biol.,1, 184 (1967).Google Scholar
  10. 10.
    V. A. Spivac, V. V. Sherbukin, V. N. Orlov, and J. M. Varshawsky, Anal. Biochem.,39, 271 (1971).CrossRefGoogle Scholar
  11. 11.
    W. R. Gray, in: C. H. W. Hirs (ed.), Methods in Enzymology, Vol. 11, Academic Press, New York (1967), p. 139.Google Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • A. N. Bulanova
  • E. P. Yulikova
  • I. A. Kashirin

There are no affiliations available

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