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Chemistry of Natural Compounds

, Volume 16, Issue 1, pp 92–95 | Cite as

Study of the total histone of the cotton plant of variety 108-F

  • T. P. Kopitsya
  • N. I. Koryakina
  • G. F. Kasymova
  • V. K. Burichenko
Article
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Abstract

From two-day etiolated shoots of the cotton plant of variety 108-F we have isolated the total histone and purified it on a column of CM-cellulose. It has been shown by gel electrophoresis and amino acid analysis that the total histone of the cotton plant, like the total histone of wheat, consists of five main fractions, the amount of the lysine-rich fraction A1 being greater in the cotton plant than in wheat (23% and 19%, respectively), and the amount of arginine-rich fraction A4 being less (19.2 and 24.3%, respectively). The total histone of the cotton plant is less basic (ratio of basic amino acids to acidic amino acids in the cotton-plant histone 1.24, in wheat histone 1.9, and in calf thymus histone 1.78) but richer in lysine as compared with arginine than the histones of wheat and calf thymus.

Keywords

Organic Chemistry Lysine Arginine Amino Acid Analysis Cotton Plant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature cited

  1. 1.
    L. V. Gofshtein, Biokhimiya,43, 947 (1978).Google Scholar
  2. 2.
    V. G. Alfrey, V. G. Littay, and A. E. Mirsky, J. Cell. Biol.,21, 213 (1964).Google Scholar
  3. 3.
    M. O. Krause and G. S. Stein, Exp. Cell. Res.,100, 63 (1976).Google Scholar
  4. 4.
    Yu. A. Tyrsin and I. A. Krasheninnikov, Lab. Delo,10, 618 (1975).Google Scholar
  5. 5.
    L. V. Gofshtein, V. I. Safonov, and N. M. Sisakyan, Dokl. Akad. Nauk SSSR,167, 1168 (1966).Google Scholar
  6. 6.
    A. P. Ibragimov, Sh. Yunushkhanov, M. P. Ibragimkhodzhaeva, B. Abdurakhmanova, and G. B. Saidova, Dokl. Akad. Nauk UzSSR, No. 2, 53 (1975).Google Scholar
  7. 7.
    S. A. Rakhmankulov, R. Abidov, and E. G. Zapruder, Uzb. Biol. Zh., No. 2, 39 (1976).Google Scholar
  8. 8.
    U. D. Barett and E. W. Johns, J. Chromatogr.,75, 161 (1973).Google Scholar
  9. 9.
    M. Tazal and R. D. Cole, J. Biol. Chem.,252, No. 12, 4068 (1977).Google Scholar
  10. 10.
    V. V. Sidorova, T. P. Kopitsya, G. F. Kasymova, N. I. Koryakina, V. K. Burichenko, and V. G. Konarev, Dokl. Akad. Nauk TadzhSSR,21, No. 4, 31 (1978).Google Scholar
  11. 11.
    E. A. Britikov, The Biological Role of Proline [in Russian], Moscow (1975).Google Scholar
  12. 12.
    T. Devenyi and J. Gergely, Amino Acids, Peptides and Proteins, Elsevier, New York (1974).Google Scholar
  13. 13.
    S. Panyim and R. Chalkey, Arch. Biochem. Biophys.,130, 337 (1969).Google Scholar
  14. 14.
    H. Busch, Histones and Other Nuclear Proteins, Academic Press, New York (1965).Google Scholar
  15. 15.
    L. V. Gofshtein, Biokhimiya,32, No. 5, 959 (1967).Google Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • T. P. Kopitsya
  • N. I. Koryakina
  • G. F. Kasymova
  • V. K. Burichenko

There are no affiliations available

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