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Chemistry of Natural Compounds

, Volume 16, Issue 6, pp 587–590 | Cite as

Characteristics of the 5S rRNA from cotton seeds

  • A. M. Érmatov
  • I. T. Yakubov
  • R. N. Nuriddinov
Article

Abstract

The individuality of the 5S rRNA from cotton seeds has been determined by the production of a single narrow band on gel electrophoresis and by the characteristic UV spectrum with values of the ratios E260/E230 and E260/E280 of 2.03–2.1 and 2.08–2.14, respectively. The melting curve of the 5S rRNA has been obtained and a total hyperchromic effect of 25% has been calculated. The nucleotide composition of the 5S rRNA has been established by determining the amount of nucleotides in an alkaline hydrolysate (22.6% of AMP; 30.2% of GMP; 22.6% of UMP; and 24.6% of CMP). The nucleotides were identified from the position of their appearance in the elution profile, from their UV spectra in 0.1 N HC1 and 0.1 KOH, and also by one- and two-dimensional chromatography of the nucleotides obtained, in the presence of markers.

Keywords

Elution Profile Cotton Seed Nucleotide Composition Isobutyric Acid Hyperchromic Effect 

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Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • A. M. Érmatov
  • I. T. Yakubov
  • R. N. Nuriddinov

There are no affiliations available

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