Chemistry of Natural Compounds

, Volume 6, Issue 1, pp 42–47 | Cite as

20α-Hydroxysteroid oxidoreductase fromActinomyces roseochromogenus ATCC 3347. Industion, primary purification, substrate specificity

  • É. A. Elin
  • L. M. Kogan
  • O. S. Tarasov
  • I. V. Torgov


The formation of 20α-hydroxysteroid oxidoreductase with a constitutive enzyme ofActinomyces roseochromogenus is activated by certain predominantly monohydroxy derivatives of pregnane. Some steroids suppress the formation of the enzyme. Intact cells ofA. roseochromogenus and a cell-free enzyme system possess identical substrate specificities which are close to the substrate specificity of the 20α-hydroxysteroid oxidoreductase of rat testes and reduce only 20-oxopregnanes with a 17-oxygen function. The specific role of the 17-oxogen function appears at the substrate-enzyme level.


NADP Ammonium Sulfate Pregnane Hydroxyprogesterone Constitutive Enzyme 


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Copyright information

© Consultants Bureau 1972

Authors and Affiliations

  • É. A. Elin
    • 1
  • L. M. Kogan
    • 1
  • O. S. Tarasov
    • 1
  • I. V. Torgov
    • 1
  1. 1.Institute of the Chemistry of Natural Compounds, AS USSRUSSR

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