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Chemistry of Natural Compounds

, Volume 2, Issue 4, pp 224–229 | Cite as

Mechanism of the inactivation of the antibiotic polymyxin M

  • L. A. Baratova
  • G. S. Katrukha
  • A. B. Silaev
Article

Summary

1. A method for the separation and quantitative determination of α- and ω-DNP derivatives of α, γ-DABA, ornithine, and lysine has been proposed.

2. A quantitative evaluation of the Nα ⇄ Nγ migration in four peptides of α, γ-DABA has been carried out.

3. The degree of migration in inactive polymyxin M and the DNP-peptides from a partial hydrolyzate of the inactivated antibiotic has been determined.

4. The amino acid composition of the DNP peptide B1 from a partial hydrolyzate of active DNP-polymyxin M has been established.

5. The quantitative aspect of the conversion of\({\text{D}}{\text{A}}\) and\({\text{g}}{\text{A}}\) diketopiperazines has been studied.

Keywords

Polymyxin Sodium Citrate Buffer Partial Hydrolyzate Percentage Degradation Diketopiperazine 

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Copyright information

© The Faraday Press, Inc 1967

Authors and Affiliations

  • L. A. Baratova
    • 1
  • G. S. Katrukha
    • 1
  • A. B. Silaev
    • 1
  1. 1.Lomonosov Moscow State UniversityUSSR

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