Summary
1. Under certain conditions, lipase immobilized on cellulose-based ion-exchange materials can be obtained. The best adsorbent is DEAE-cellulose.
2. The optimum conditions for the immobilization of lipase on DEAE-cellulose are pH 8.0, time of contact with the support 15–20 min, temperature 10° C.
3. The immobilized lipase (DEAE-cellulose-lipase) retains the main functional properties of the native enzyme.
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Literature cited
K. Davranov and M. Rizaeva, Khim. Prirodn. Soedin., 279 (1975).
K. Davranov, M. Z. Zakirov, and M. I. Rizaeva, Khim. Prirodn. Soedin., 636 (1976).
A. I. Kestner and M. I. Kreén, The Production and Use of Immobilized Enzymes [in Russian], ÉstNIINTI, Tallin (1973).
Additional information
Department of Microbiology, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 267–271, March–April, 1977.
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Davranov, K.D., Rizaeva, M., Zakirov, M.Z. et al. Preparation of adsorbed lipase and its properties. Chem Nat Compd 13, 228–231 (1977). https://doi.org/10.1007/BF00563955
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DOI: https://doi.org/10.1007/BF00563955