Isolation and purification of L-asparaginase from Escherichia coli 671
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A modified method for obtaining purified L-asparaginase fromE. coli 671 is proposed. The purification includes extraction with water from an acetone powder, acid-thermal treatment, fractionation with acetone and with polyethyleneglycol, and volume chromatography on DEAE-cellulose.
The specific activity of the L-asparaginase was 200 MU/mg of protein, and the yield of enzyme 19.6%. Its purity was shown by electrophoresis in polyacrylamide gel.
KeywordsPotassium Phosphate Buffer Polyethyleneglycol Cold Acetone Acetone Powder Disk Electrophoresis
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