Isolation and purification of L-asparaginase from Escherichia coli 671
A modified method for obtaining purified L-asparaginase fromE. coli 671 is proposed. The purification includes extraction with water from an acetone powder, acid-thermal treatment, fractionation with acetone and with polyethyleneglycol, and volume chromatography on DEAE-cellulose.
The specific activity of the L-asparaginase was 200 MU/mg of protein, and the yield of enzyme 19.6%. Its purity was shown by electrophoresis in polyacrylamide gel.
KeywordsPotassium Phosphate Buffer Polyethyleneglycol Cold Acetone Acetone Powder Disk Electrophoresis
- 4.A. Arens, E. Rauenbusch, E. Irion, et al., Z. Physiol. Chem.,197, 351 (1970).Google Scholar
- 5.S. V. Mardashev, E. A. Kozlov, N. N. Sokolov, I. V. Kobachevich, and L. V. Gorbatenko, Vopr. Med. Khimii,17, 318 (1972).Google Scholar
- 6.R. A. Zhagat, I. K. Shprunka, I. A. Éiduze, I. A. Vina, and D. Ya. Daiya, Izd. Akad. Nauk LatvSSR, Ser. Khim., No. 1, 73 (1972).Google Scholar