Immunogenetics

, Volume 22, Issue 2, pp 159–167 | Cite as

Activation of T lymphocytes results in an increase inH-2-encoded neuraminidase

  • Nicholas F. Landolfi
  • Joseph Leone
  • James E. Womack
  • Richard G. Cook
Article

Abstract

The endogenous neuraminidase activity of various mouse lymphoid subpopulations and tissue compartments was examined by a sensitive fluorometric assay. These analyses indicated that activated T lymphocytes possessed a significantly higher level of intracellular neuraminidase than activated B or resting T or B lymphocytes. Examination of the level of neuraminidase in bone marrow, thymus, lymph node, and unfractionated spleen indicated that these lymphoid tissues contained significantly less neuraminidase than was detected in stimulated T cells. Kinetic studies revealed that the majority of the increase in neuraminidase activity occurred between 24 and 48 h following stimulation. Analysis of activated T lymphocytes prepared from a panel of inbred mouse strains indicated that cells from mice of theH-2 v haplotype, which possess theNeu-1a allele and are deficient in liver neuraminidase, exhibited a level of activity which was significantly lower than that detected in stimulated T cells from other mouse strains. These results indicate that the endogenous neuraminidase activity of T lymphocytes increases upon stimulation, and that the level of this enzyme activity in lymphoid cells is also controlled by theNeu-1 locus, which is located in theH-2 region of the major histocompatibility complex.

Keywords

Lymph Node Bone Marrow Enzyme Activity Major Histocompatibility Complex Kinetic Study 

Abbreviations used in this paper

MHC

major histocompatibility complex

LPS

lipopolysaccharide

DXS

dextran sulfate

IL-2

interleukin 2

NANA

N-acetylneuraminic acid

sIg

surface immunoglobulin

Con A

concanavalin A

C57BL/10

B10

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Nicholas F. Landolfi
    • 1
  • Joseph Leone
    • 1
  • James E. Womack
    • 2
  • Richard G. Cook
    • 1
  1. 1.Howard Hughes Medical Institute and the Department of Microbiology and ImmunologyBaylor College of MedicineHouston
  2. 2.Department of Veterinary PathologyTexas A & M UniversityCollege Station

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