, Volume 22, Issue 2, pp 159–167 | Cite as

Activation of T lymphocytes results in an increase inH-2-encoded neuraminidase

  • Nicholas F. Landolfi
  • Joseph Leone
  • James E. Womack
  • Richard G. Cook


The endogenous neuraminidase activity of various mouse lymphoid subpopulations and tissue compartments was examined by a sensitive fluorometric assay. These analyses indicated that activated T lymphocytes possessed a significantly higher level of intracellular neuraminidase than activated B or resting T or B lymphocytes. Examination of the level of neuraminidase in bone marrow, thymus, lymph node, and unfractionated spleen indicated that these lymphoid tissues contained significantly less neuraminidase than was detected in stimulated T cells. Kinetic studies revealed that the majority of the increase in neuraminidase activity occurred between 24 and 48 h following stimulation. Analysis of activated T lymphocytes prepared from a panel of inbred mouse strains indicated that cells from mice of theH-2 v haplotype, which possess theNeu-1a allele and are deficient in liver neuraminidase, exhibited a level of activity which was significantly lower than that detected in stimulated T cells from other mouse strains. These results indicate that the endogenous neuraminidase activity of T lymphocytes increases upon stimulation, and that the level of this enzyme activity in lymphoid cells is also controlled by theNeu-1 locus, which is located in theH-2 region of the major histocompatibility complex.


Lymph Node Bone Marrow Enzyme Activity Major Histocompatibility Complex Kinetic Study 

Abbreviations used in this paper


major histocompatibility complex




dextran sulfate


interleukin 2


N-acetylneuraminic acid


surface immunoglobulin

Con A

concanavalin A




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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Nicholas F. Landolfi
    • 1
  • Joseph Leone
    • 1
  • James E. Womack
    • 2
  • Richard G. Cook
    • 1
  1. 1.Howard Hughes Medical Institute and the Department of Microbiology and ImmunologyBaylor College of MedicineHouston
  2. 2.Department of Veterinary PathologyTexas A & M UniversityCollege Station

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