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Mouse carbonic anhydrase III: Nucleotide sequence and expression studies

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Abstract

A cDNA for the mouse carbonic anhydrase, CAIII, has been isolated from a λgt11 expression library. The cloned cDNA contains all of the coding region (777 bp) and both 5′ untranslated (86-bp) and 3′ untranslated (217-bp) sequences. The coding sequence shows 87% homology at the nucleotide level and 91% homology, when amino acid residues are compared, with human CAIII. Protein and mRNA analyses show that CAIII is present at low levels in cultured myoblasts and is abundant in adult skeletal muscle and in liver. The marked sex-related differences in CAIII distribution, described for rat liver, are not seen in the mouse. Restriction fragment length polymorphisms usingTaqI andPstI are described which distinguish betweenMus spretus andMus musculus domesticus.

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Authors and Affiliations

  1. MRC Human Biochemical Genetics Unit, The Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, U.K.

    Susan Tweedie & Yvonne Edwards

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  1. Susan Tweedie
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  2. Yvonne Edwards
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S. T. is supported by an MRC postgraduate training award.

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Tweedie, S., Edwards, Y. Mouse carbonic anhydrase III: Nucleotide sequence and expression studies. Biochem Genet 27, 17–30 (1989). https://doi.org/10.1007/BF00563015

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  • DOI: https://doi.org/10.1007/BF00563015

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