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Plasma levels of triamcinolone acetonide as determined by radioimmunoassay after topical application

Archives of Dermatological Research volume 259, pages 63–70 (1977)Cite this article

Summary

In this paper a method for the radioimmunological determination of triamcinolone acetonide (TAAc) in plasma is described. The antiserum used in the radioimmunological determinations showed high specifity for TAAc; the minimal detectable amount was 0.2 ng. The course of the plasma concentrationtime curve of TAAc after intravenous injection into rats was analysed.

The concentration of TAAc in the blood of the patients after topical application of the drug is very low. No detectable amount of TAAc was found in the blood of out-patients. Hospitalized patients treated with TAAc under plastic occlusion showed plasma levels ranging from 0.8 to 3.4 ng/ml. This level remained constant throughout the treatment.

Zusammenfassung

Es wird eine radioimmunologische Methode zur Bestimmung von Triamcinolonacetonid (TAAc) im Plasma beschrieben. Das für diese Methode verwandte Antiserum zeigte hohe Spezifität für TAAc. Die Empfindlichkeit der Methode ging bis 0,2 ng. Der Verlauf der Plasmakonzentrationskurve des TAAc nach intravenöser Injektion in Ratten wurde bestimmt.

Die Konzentration des TAAc im Blut der Patienten nach lokaler Anwendung ist sehr gering. Im Blut von ambulanten Patienten wurden keine TAAc-Mengen aufgefunden. Hospitalisierte Patienten zeigten unter TAAc-Plastikokklusionsverband Plasmaspiegel, die sich zwischen 0,8 bis 3,4 ng/ml bewegten. Diese Spiegel blieben konstant für die gesamte Behandlungsdauer.

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Affiliations

  1. Departments of Dermatology, Leiden, The Netherlands

    Maria Ponec & Ada de Lijster

  2. Pathological Chemistry, University Hospital, Rijnsburgerweg 10, Leiden, The Netherlands

    Marijke Frölich & A. J. Moolenaar

Authors
  1. Maria Ponec
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  2. Marijke Frölich
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  3. Ada de Lijster
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  4. A. J. Moolenaar
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Ponec, M., Frölich, M., de Lijster, A. et al. Plasma levels of triamcinolone acetonide as determined by radioimmunoassay after topical application. Arch. Derm. Res. 259, 63–70 (1977). https://doi.org/10.1007/BF00562738

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  • DOI: https://doi.org/10.1007/BF00562738

Keywords

  • Public Health
  • Plasma Level
  • Intravenous Injection
  • High Specifity
  • Topical Application