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Archiv für dermatologische Forschung

, Volume 251, Issue 3, pp 205–220 | Cite as

Human-melanom FUHS-1

Beschreibung einer neuen Dauerzellinie
  • V. Misgeld
  • Chr Meisel
Article
  • 18 Downloads

Zusammenfassung

Vorgestellt wird eine neue Dauerzellinie, die aus einem malignen Melanom, entstanden auf dem Boden einer melanotischen Präkanzerose im Unterschenkelbereich, gezüchtet wurde. Nach bis jetzt 31 Monaten wurde die 105. Subkultur erreicht. Als Medium der Primärkultur diente TC Medium Eagle spinner modified, ab dann wurde Medium MEM/Hanks/0.35 g/l NaHCO3 und ab der 6. Subkultur parallel NCTC 135 — jeweils mit Zusätzen — angewendet. Die Zellen wachsen als Monolayer, die absolute Aussaateffektivität der Dauerzelllinie liegt bei 80%, zu beobachten sind überwiegend epitheloide und dendritische melaninproduzierende Zellen. Die Chromosomenzahl schwankt erheblich, der Karyotyp der Dauerzelllinie liegt im subtriploiden Bereich. Ein Markerchromosom mit submedianer Lage des Centromers ist größenmäßig der Gruppe (A)I zuzuordnen.

Human melanoma FUHS-1

Description of a New Cell Line

Summary

Presented is a new established cell line, which was cultivated from a melanoma originating from a melanosis praeblastomatosa circumscripta of the leg. Recently subculture No. 105 was reached after a period of 31 month. Medium of primary culture was TC Medium Eagle spinner modified, followed from subculture No. 1 by MEM/Hanks/0.35 g/l NaHCO3. From subculture No. 6 NCTC 135 was applied simultaneously—each respective supplements. The cells grow as monolayer; absolute plating efficiency of the established cell line lies by 80%; epithelial-like and dendritic melanin-producing cells are prominent. The number of chromosomes is considerably fluctuating; the karyotype of the established cell line lies within the range of subtriploidy. Sizewise a marker in submedian position of centromer is to be compared with one of group (A)I.

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • V. Misgeld
    • 1
  • Chr Meisel
    • 1
  1. 1.Klinikum Steglitz, Hautklinik und PoliklinikFreie Universität BerlinGermany

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