Skip to main content
Log in

Studies on rat hair cultures

III. The Effects of Chondroitinsulfate

  • Published:
Archiv für dermatologische Forschung Aims and scope Submit manuscript

Summary

The authors have developed a technique to quantitatively assess follicle growth in culture. Rat hair follicles increase in volume when chondroitinsulfate A, but not B and C, is added to the culture medium.

Zusammenfassung

Es wurde eine Methode entwickelt, mit deren Hilfe das in vitro-Wachstum von Haarfollikeln quantitativ gemessen werden kann. Rattenhaarfollikel vergrößern das Organvolumen bei Zusatz von Chondroitinsulfat A, nicht aber B oder C zum Kulturmedium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bartosova, L., Rebora, A., Moretti, G., Cipriani, C.: Studies on rat hair cultures. I. A. reevaluation of the technique. Arch. Derm. Forsch.240, 95–106 (1971)

    Google Scholar 

  2. Bentley, J. P.: The biological role of the ground substance mucopolysaccharides. The dermis, p. 103, W. Montagnaet al., eds. New York: Academic Press 1970

    Google Scholar 

  3. Cairns, R. J.: Metabolic and nutritional disorders. Textbook of Dermatology, p. 1619, D. S. Rooket al., eds. Oxford: Blackwell 1968

    Google Scholar 

  4. Crovato, F., Moretti, G., Bertamino, R.: Histochemistry of dermis and blood vessels in male pattern alopecia. Biopathology of pattern alopecia, pp. 191–199, A. Baccaredda-Boy and G. Moretti, eds. Basel: Karger 1968

    Google Scholar 

  5. McKusick, V. A.: Heritable disorders of connective tissue with skin changes. Dermatology in General Medicine, p. 1226, T. B. Fitzpatricket al., eds. New York: McGraw Hill 1971

    Google Scholar 

  6. Meyer, K., Kaplan, D., Steigleder, G. K.: Effect of AMPS on hair growth in the rabbit. Proc. Soc. exp. Biol. (N.Y.)108, 59–63 (1961)

    Google Scholar 

  7. Montagna, W.: The structure and function of skin, p. 257. New York: Academic Press 1962

    Google Scholar 

  8. Montagna, W., Chase, J. D., Malone, J. D., Melaragno, H. P.: Cyclic changes in polysaccharides of the papilla of the hair follicle. Quart. J. Microscop. Sci.93, 241–245 (1952)

    Google Scholar 

  9. Moretti, G., Cipriani, C., Rebora, A., Rampini, E., Crovato, F.: Correlation of tissue AMPS with the hair cycle. J. invest. Derm.48, 498–503 (1967)

    Google Scholar 

  10. Moretti, G., Giacometti, C., Boido, V., Rebora, A.: Histamine, serotonin and mast-cells in the skin of rat during the hair cycle. J. invest. Derm.40, 205–212 (1963)

    Google Scholar 

  11. Morrison, L. M., Murata, K., Quilligan, J. J., Jr., Schjeide, O. A., Freeman, L.: Growth stimulating effects of AMPS. Proc. Soc. exp. Biol. (N.Y.)118, 770–772 (1965)

    Google Scholar 

  12. Rebora, A., Moretti, G.: Studies on rat hair cultures. II. The effect of histamine. Arch. Derm. Forsch.242, 323–328 (1972)

    Google Scholar 

  13. Ryan, T. J.: The direction of growth of epithelium. Brit. J. Derm.78, 403–415 (1966)

    Google Scholar 

  14. Schiller, S., Dorfman, A.: Biosynthesis of AMPS in the skin of alloxan-diabetic rats. Biochim. biophys. Acta (Amst.)16, 304 (1955): quoted by Z. Stary: Chemistry of the Cutis. Hdb. Haut- u. Geschl.-Kr., p. 676. A. Marchionini and H. W. Spier, eds., vol. I/3. Berlin-Heidelberg-New York: Springer 1963

    Google Scholar 

  15. Schiller, S., Slover, G. A., Dorfman, A.: A method for the separation of AMPS. Its application to the isolation of heparin from the skin of rats. J. biol. Chem.236, 983–987 (1961)

    Google Scholar 

  16. Slack, H. G. B.: Connective tissue growth stimulated by carageenin. III. The nature and amount of polysaccharide produced in normal and scorbutic guinea pigs and the metabolism of a CSA fraction. Biochem. J.69, 125 (1958). Quoted by Z. Stary: Chemistry of the cutis. Hdb. Haut- u. Geschl.-Kr., p. 682, A. Marchionini and H. W. Spier, eds., Vol. I/3. Berlin-Heidelberg-New York: Springer 1963

    Google Scholar 

  17. Takaki, Y., Guarrera, M., Rebora, A., Moretti, G., unpublished data.

  18. Van Lis, J. M. J., Kruiswijk, T., Mager, W. H., Kalsbeck, G. L.: Glycosaminoglycans in human skin. Brit. J. Derm.88, 335–361 (1973)

    Google Scholar 

  19. Winter, V.: Fluorezentzmikroskopische Studien der menschlichen embryonalen Haut. I. Entwicklung des menschlichen Haares. Dermatologia (Basel)136, 29–34 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Partly supported by Hoffmann-La Roche, Ltd., Basle, Switzerland.

Presented at the joint ESDR-SID Meeting in Amsterdam, 1972.

Reprints are to be requested to G. Moretti, Clinica Dermatologica dell'Università di Genova, Genova, Italy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takaki, Y., Guarrera, M., Rebora, A. et al. Studies on rat hair cultures. Arch. Derm. Forsch. 251, 61–68 (1974). https://doi.org/10.1007/BF00561712

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00561712

Keywords

Navigation