Summary
The effect of prostaglandin E1 (PGE1) on growth of mouse epidermis has been studiedin vitro. Preliminary experiments showed that PGE1 20 µg/ml caused an increase in H3-thymidine labelling index after 1 hr and an increase in thickness and epidermal cell counts after 4 hrs culture. The uptake of H3-thymidine and change in total amount of DNA and protein were then determined using isolated cell cultures. Incubation of cell cultures with PGE1 0.1–20 µg/ml for 1 and 4 hrs resulted in increased uptake of H3-thymidine, DNA synthesis and protein synthesis.
Zusammenfassung
Der Einfluß von Prostaglandin E1 (PGE1) auf das Wachstum von Mäuseepidermis wurde in vitro untersucht. Vorläufige Ergebnisse zeigten, daß PGE1, 20 µg/ml, eine Erhöhung des3H-Thymidin-Markierungsindexes nach 1 Std hervorrief. Nach 4-stündiger Kulturdauer waren die Dicke der Epidermis und die Zellzahl erhöht. Im Anschluß an diese Befunde wurden3H-Thymidin-Einbau, Gesamt-DNS und Proteingehalt in isolierten Zellkulturen bestimmt. Die Inkubation der Zellkulturen mit PGE1, 0.1–20 µg/ml über 1 und 4 Std, rief eine Erhöhung des3H-Thymidin-Einbaus, der DNS-Synthese und der Proteinsynthese hervor.
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References
Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J.62, 315–323 (1956)
Greaves, M. W., McDonald-Gibson, W. J.: Prostaglandin biosynthesis by human skin and its inhibition by corticosteroids. Brit. J. Pharmacol.46, 172–175 (1972)
Jessup, S. J., McDonald-Gibson, W. J., Ramwell, P. W., Shaw, J. E.: Biosynthesis and release of prostaglandins on hormonal treatment of frog skin, and their effect on ion transport. Fed. Proc.29, 287 (1970)
Kischer, C. W.: Effect of specific prostaglandins on development of chick embryo skin and down feather organ “in vitro”. Develop. Biol.16, 203–215 (1967)
Kischer, C. W.: Accelerated maturation of chick embryo skin treated with prostaglandin (PGE): an electron microscope study. Amer. J. Anat.124, 491–496 (1969)
Lowry, O. H.: Protein measurement with the Folin: Phenol reagent. J. biol. Chem.193, 265–275 (1951)
Robison, G. A., Butcher, R. W., Sutherland, E. W.: Cyclic AMP, pp. 385–388. New York-London: Academic Press 1971
Van Dorp, D.: Recent developments in the biosynthesis and analysis of prostaglandins. Ann. N.Y. Acad. Sci.180–199 181 (1971)
Voorhees, J. J., Duell, E. A.: Psoriasis, as a possible defect of the Adenyl cyclase-cyclic AMP Cascade. Arch. Derm.104, 352–358 (1971)
Ziboh, V. A.: Biosynthesis of prostaglandin E2 in human skin: subcellular localization and inhibition by unsaturated fatty acids and anti-inflammatory drugs. J. Lipid Res.14, 377–384 (1973)
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Bem, J.L., Greaves, M.W. Prostaglandin E1 effects on epidermal cell growth “in vitro”. Arch. Derm. Forsch. 251, 35–41 (1974). https://doi.org/10.1007/BF00561708
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DOI: https://doi.org/10.1007/BF00561708