Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations ofEst-5, Icd-1, Acp-1, andPgd-1. The locations ofAmp-1, Amp-2, Amp-4, Pox-5, Got-1, andCat-1 were further confirmed from segregation data in BC1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered forAmp-1 andAmp-2. On the basis of these dataGot-1, Est-5, andIcd-1 were located to chromosome 1,Amp-1 to chromosome 2,Cat-1 andPox-5 to chromosome 3,Acp-1 to chromosome 6,Amp-2 andAmp-4 to chromosome 8, andPgd-1 to chromosome 11. BecauseAcp-2 andPox-2 are known to be linked withAcp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Allard, R. W. (1956). Formulas and tables to facilitate the calculation of recombination values in heredity.Hilgardia 2235.
Chu, Y. E. (1967). Variations in peroxidase isozymes ofOryza perennis andO. sativa.Jap. J. Genet. 42233.
Fobes, J. F. (1980). Trisomic analysis of isozymic loci in tomato species: Segregation and dosage effect.Biochem. Genet. 18401.
Fu, P. Y., and Pai, C. (1979). Genetic studies on isozymes in rice plant. II. Classification and geographical distribution of cultivated rice through isozyme studies.J. Agr. Assoc. China 1071 (Chinese with English summary).
Glaszmann, J. C. (1985). A varietal classification of Asian cultivated rice (Oryza sativa L.) based on isozyme polymorphism. InRice Genetics International Rice Research Institute, Los Baños, Laguna, Philippines, pp. 83–90.
Glaszmann, J. C., Benoit, H., and Arnaud, M. (1984). Classification des riz cultivès (Oryza sativa L.) Utilisation de la variabilitè isoenzymatique.L'Agron. Trop. 39(1):51.
Khush, G. S., Singh, R. J., Sur, S. C., and Librojo, A. L. (1984). Primary trisomics of rice: Origin, morphology, cytology and use in linkage mapping.Genetics 107141.
Nakagahra, M. (1977). Genic analysis for esterase isoenzymes in rice cultivars.Jap. J. Breed. 27141.
Nakagahra, M. (1978). The differentiation, classification, and center of genetic diversity of cultivated rice (Oryza sativa L.) by isozyme analysis. Trop. Agr. Res. Ser. No. 11, Japan.
Nakagahra, M., and Hayashi, K. I. (1976). Detection of esterase isozyme loci ofOryza sativa L.Jap. J. Breed. 26 (Suppl. 1):114.
Nakagahra, M., Akihama, T., and Hayashi, K. I. (1975). Genetic variation and geographic cline of esterase isozymes in native rice varieties.Jap. J. Genet. 50373.
Nielsen, G., and Frydenberg, O. (1971). Chromosome location of the esterase lociEst-1 andEst-2 in barley by means of trisomics.Hereditas 67152.
Nielsen, G., and Scandalios, J. G. (1974). Chromosomal location by use of trisomics and new alleles of an endopeptidase inZea mays.Genetics 77679.
Pai, C., Endo, T., and Oka, H. I. (1973). Genic analysis for peroxidase isozymes and their organ specificity inO. perennis andO. sativa.Can. J. Genet. Cytol. 15845.
Pai, C., Endo, T., and Oka, H. I. (1975). Genic analysis for acis phosphatase isozymes inO. perennis andO. sativa.Can. J. Genet. Cytol. 47637.
Ranjhan, S.,et al. (1988). Chromosomal location of four isozyme loci by trisomic analysis in rice (Oryza sativa L.).Theor. Appl. Genet. (in press).
Sano, R., and Barbier, P. (1985). Analysis of five isozyme genes and chromosomal locations ofAmp-1.Rice Genet. Newslett. 260.
Sano, R., and Morishima, H. (1984). Linkage relationships among 6 polymorphic isozyme loci in rice cultivars.Jap. J. Breed. 34 (Suppl. 11):252.
Second, G. (1982). Origin of the genic diversity of cultivated rice (Oryza spp.): Study of the polymorphism scored at 40 isozyme loci.Jap. J. Genet. 5725.
Second, G. (1985). Evolutionary relationships in theSativa groups ofOryza based on isozyme data.Gènèt. Sèl. Evol. 17(1):89.
Shahi, B. B., Chu, Y. E., and Oka, H. I. (1968). Analysis of genes controlling peroxidase isozymes inOryza sativa andO. perennis.Jap. J. Genet. 44321.
Shahi, B. B., Morishima, H., and Oka, H. I. (1969). A survey of variations in peroxidase, acid phosphatase and esterase isozymes of wild and cultivatedOryza species.Jap. J. Genet. 44303.
Tanksley, S. D. (1979). Linkage, chromosomal association, and expression ofAdh-1 andPgm-2 in tomato.Biochem. Genet. 171159.
Tanksley, S. D., and Rick, C. M. (1980). Isozymic linkage map of the tomato: Applications in genetics and breeding.Theor. Appl. Genet. 157161.
Trouslot, P., and Second, G. (1980). Technique d'èlectrophorèse en gel d'amidon appliquèe à l'ètude du polymorphisme de quatorze enzymes du riz. InElectrophorèse d'enzymes de riz Travaux et Documents de l'Orstom, 120, Orstom, Paris.
The senior author wishes to acknowledge the financial support from the Chinese government.
About this article
Cite this article
Wu, KS., Glaszmann, JC. & Khush, G.S. Chromosomal locations of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis. Biochem Genet 26, 303–320 (1988). https://doi.org/10.1007/BF00561468
- linkage relations
- chromosome maps