Biochemical Genetics

, Volume 26, Issue 3–4, pp 303–320 | Cite as

Chromosomal locations of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis

  • Kun-Sheng Wu
  • Jean-Christophe Glaszmann
  • Gurdev S. Khush


Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F1 trisomics. This served as the primary source of evidence for chromosome locations ofEst-5, Icd-1, Acp-1, andPgd-1. The locations ofAmp-1, Amp-2, Amp-4, Pox-5, Got-1, andCat-1 were further confirmed from segregation data in BC1 generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered forAmp-1 andAmp-2. On the basis of these dataGot-1, Est-5, andIcd-1 were located to chromosome 1,Amp-1 to chromosome 2,Cat-1 andPox-5 to chromosome 3,Acp-1 to chromosome 6,Amp-2 andAmp-4 to chromosome 8, andPgd-1 to chromosome 11. BecauseAcp-2 andPox-2 are known to be linked withAcp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.

Key words

isozymes trisomics linkage relations chromosome maps 


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  1. Allard, R. W. (1956). Formulas and tables to facilitate the calculation of recombination values in heredity.Hilgardia 2235.Google Scholar
  2. Chu, Y. E. (1967). Variations in peroxidase isozymes ofOryza perennis andO. sativa.Jap. J. Genet. 42233.Google Scholar
  3. Fobes, J. F. (1980). Trisomic analysis of isozymic loci in tomato species: Segregation and dosage effect.Biochem. Genet. 18401.Google Scholar
  4. Fu, P. Y., and Pai, C. (1979). Genetic studies on isozymes in rice plant. II. Classification and geographical distribution of cultivated rice through isozyme studies.J. Agr. Assoc. China 1071 (Chinese with English summary).Google Scholar
  5. Glaszmann, J. C. (1985). A varietal classification of Asian cultivated rice (Oryza sativa L.) based on isozyme polymorphism. InRice Genetics International Rice Research Institute, Los Baños, Laguna, Philippines, pp. 83–90.Google Scholar
  6. Glaszmann, J. C., Benoit, H., and Arnaud, M. (1984). Classification des riz cultivès (Oryza sativa L.) Utilisation de la variabilitè isoenzymatique.L'Agron. Trop. 39(1):51.Google Scholar
  7. Khush, G. S., Singh, R. J., Sur, S. C., and Librojo, A. L. (1984). Primary trisomics of rice: Origin, morphology, cytology and use in linkage mapping.Genetics 107141.Google Scholar
  8. Nakagahra, M. (1977). Genic analysis for esterase isoenzymes in rice cultivars.Jap. J. Breed. 27141.Google Scholar
  9. Nakagahra, M. (1978). The differentiation, classification, and center of genetic diversity of cultivated rice (Oryza sativa L.) by isozyme analysis. Trop. Agr. Res. Ser. No. 11, Japan.Google Scholar
  10. Nakagahra, M., and Hayashi, K. I. (1976). Detection of esterase isozyme loci ofOryza sativa L.Jap. J. Breed. 26 (Suppl. 1):114.Google Scholar
  11. Nakagahra, M., Akihama, T., and Hayashi, K. I. (1975). Genetic variation and geographic cline of esterase isozymes in native rice varieties.Jap. J. Genet. 50373.Google Scholar
  12. Nielsen, G., and Frydenberg, O. (1971). Chromosome location of the esterase lociEst-1 andEst-2 in barley by means of trisomics.Hereditas 67152.Google Scholar
  13. Nielsen, G., and Scandalios, J. G. (1974). Chromosomal location by use of trisomics and new alleles of an endopeptidase inZea mays.Genetics 77679.Google Scholar
  14. Pai, C., Endo, T., and Oka, H. I. (1973). Genic analysis for peroxidase isozymes and their organ specificity inO. perennis andO. sativa.Can. J. Genet. Cytol. 15845.Google Scholar
  15. Pai, C., Endo, T., and Oka, H. I. (1975). Genic analysis for acis phosphatase isozymes inO. perennis andO. sativa.Can. J. Genet. Cytol. 47637.Google Scholar
  16. Ranjhan, S.,et al. (1988). Chromosomal location of four isozyme loci by trisomic analysis in rice (Oryza sativa L.).Theor. Appl. Genet. (in press).Google Scholar
  17. Sano, R., and Barbier, P. (1985). Analysis of five isozyme genes and chromosomal locations ofAmp-1.Rice Genet. Newslett. 260.Google Scholar
  18. Sano, R., and Morishima, H. (1984). Linkage relationships among 6 polymorphic isozyme loci in rice cultivars.Jap. J. Breed. 34 (Suppl. 11):252.Google Scholar
  19. Second, G. (1982). Origin of the genic diversity of cultivated rice (Oryza spp.): Study of the polymorphism scored at 40 isozyme loci.Jap. J. Genet. 5725.Google Scholar
  20. Second, G. (1985). Evolutionary relationships in theSativa groups ofOryza based on isozyme data.Gènèt. Sèl. Evol. 17(1):89.Google Scholar
  21. Shahi, B. B., Chu, Y. E., and Oka, H. I. (1968). Analysis of genes controlling peroxidase isozymes inOryza sativa andO. perennis.Jap. J. Genet. 44321.Google Scholar
  22. Shahi, B. B., Morishima, H., and Oka, H. I. (1969). A survey of variations in peroxidase, acid phosphatase and esterase isozymes of wild and cultivatedOryza species.Jap. J. Genet. 44303.Google Scholar
  23. Tanksley, S. D. (1979). Linkage, chromosomal association, and expression ofAdh-1 andPgm-2 in tomato.Biochem. Genet. 171159.Google Scholar
  24. Tanksley, S. D., and Rick, C. M. (1980). Isozymic linkage map of the tomato: Applications in genetics and breeding.Theor. Appl. Genet. 157161.Google Scholar
  25. Trouslot, P., and Second, G. (1980). Technique d'èlectrophorèse en gel d'amidon appliquèe à l'ètude du polymorphisme de quatorze enzymes du riz. InElectrophorèse d'enzymes de riz Travaux et Documents de l'Orstom, 120, Orstom, Paris.Google Scholar

Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Kun-Sheng Wu
    • 1
  • Jean-Christophe Glaszmann
    • 2
  • Gurdev S. Khush
    • 3
  1. 1.Department of AgronomySouth China Agricultural UniversityGuangzhouChina
  2. 2.Institut de Recherches Agronomiques Tropicales et des Cultures VivrièresCentre de Cooperation Internationale en Recherche Agronomique pour le DeveloppmentMontpellier, CedexFrance
  3. 3.International Rice Research InstituteManilaPhilippines

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