Abstract
Malate dehydrogenase (MDH) band relative mobility (R m) and activity were examined in leaf extracts of Durrant's flax genotrophs, L and S, and flax genotypes, R and M. MDH activity in leaves from just below the inflorescence was higher in the two smaller, sparsely branched plant types, S and M, than in the larger, more branched plant types, L and R. The MDH electrophoretic banding pattern in flax leaf extracts consisted of three major anionic bands, MDH-1, MDH-2, and MDH-3. NoR m differences were detected between corresponding isozymes of genotypes R and M. For the genotrophs, however, all three bands of S migrated faster than the corresponding bands of L. Codominance was absent in F1 hybrids; SR m was dominant for MDH-2 and MDH-3 and LR m was dominant for MDH-1. The observations suggest that MDHR m in L and S may be controlled by a modifier locus (or loci). Previous studies indicate that a modifier locus may also control heritable genotrophic differences in peroxidase (PER) and acid phosphates (AP)R m. The three enzyme systems are compared.
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The financial assistance of the Natural Sciences and Engineering Research Council of Canada is acknowledged with thanks.
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Fieldes, M.A. Relative mobility and activity of leaf malate dehydrogenase in flax(Linum usitatissimum) genotrophs and genotypes. Biochem Genet 26, 261–275 (1988). https://doi.org/10.1007/BF00561465
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DOI: https://doi.org/10.1007/BF00561465