Abstract
Tfm Lac, a new occurrence of the X-linked mutation testicular feminization, has been isolated in a stock of mice and mapped to the same region as the originalTfm H mutation. We compared these two mutants to determine if there are differences in their putative residual androgen receptors or androgen responsiveness. Such differences have been reported forTfm mutations in humans. We found no evidence for induction of ornithine decarboxylase (ODC) activity inTfm Lac despite androgen treatment for up to 3 weeks. This is in agreement with findings forTfm H. Both of these mutants expressed small amounts of androgen binding activity which shared some properties with the normal androgen receptors in mouse kidney. The binding was distinguishable between the two mutants, however, as determined by hormone saturation experiments utilizing DNA-cellulose chromatography. These findings confirm the independence of the two mutations and are consistent with their being allelic: both result in severe deficits of androgen binding and response.
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This work was supported by NIH Grants HD17666 and HD20327 (TOF), research grants from the March of Dimes-Birth Defects Foundation (TOF), and a postdoctoral National Research Service Award (J.A.P.) in Endocrinology (AM07337).
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Politch, J.A., Fox, T.O., Houben, P. et al. Tfm Lac: A second isolation of testicular feminization in mice. Biochem Genet 26, 213–221 (1988). https://doi.org/10.1007/BF00561461
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DOI: https://doi.org/10.1007/BF00561461