Summary
Cultured human leucocytes have been used to examine the toxicity and phototoxicity of 8-methoxy psoralen (8-MOP). It has been shown that 8-MOP depresses cell turnover at concentrations of 10−10 M or above. In black light (UVA), 8-MOP inhibition begins at concentrations of 10−14 M. Patients treated for psoriasis with 8-MOP show concentrations of 10−6-10−7 M. The inhibition of cell turnover observed with the present technique is at this concentration 40% for 8-MOP alone and 70% for 8-MOP and black light. These results can be related to the micronuclei and chromosome abnormalities seen in dividing cells.
Zusammenfassung
Gezüchtete humane Leukocyten sind für die Untersuchung der Toxicität und der Phototoxicität des 8-Methoxy-Psoralen (8-MOP) verwendet worden. Man hat festgestellt, daß 8-MOP in einer Konzentration von mehr als 10−10 M die Zellteilung hemmt. Bei 8-MOP und black light (UVA) fängt die Hemmung bei 10−14 M an. Bei Patienten, die wegen Psoriasis mit 8-MOP behandelt werden, zeigen sich Konzentrationen von 10−6-10−7 M. Die mit der hier angewendeten Technik festgestellten Hemmung der Zellteilung beträgt bei dieser Konzentration für 8-MOP allein 40% und für 8-MOP + UVA 70%. Diese Ergebnisse sind mit dem Befund an Mikronuclei und Chromosomabnormalitäten in sich teilenden Zellen in Zusammenhang zu sehen.
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Wulf, H.C., Wettermark, G. Toxic Effects of 8-Methoxypsoralen on Lymphocyte Devision. Arch. Derm. Res. 260, 87–92 (1977). https://doi.org/10.1007/BF00561113
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DOI: https://doi.org/10.1007/BF00561113