Diffusion barrier to horseradish peroxidase in the vascular stria of the guinea pig

  • Finn Ø. Winther


Guinea pigs were given horseradish peroxidase intracardially and its diffusion in the vascular stria was studied. The Peroxidase spred freely among the intermediate cells and the marginal cells, but was never found to have passed zones occupied by tight junctions. It is concluded that the zones of tight junctions bordering the vascular stria represent a diffusion barrier to horseradish peroxidase.


Cochlea Vascular stria Guinea pig Diffusion barrier 


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  1. Brightman, M. V., Reese, T. S.: Junctions between intimately apposed cell membranes in the vertebrate brain. J. Cell Biol.40, 648–677 (1969).Google Scholar
  2. Duvall, A. J., Quick, C. A., Sutherland, C. R.: Horseradish peroxidase in the lateral cochlear wall. Arch. Otolaryng.93, 304–316 (1971).Google Scholar
  3. Farquhar, M. G., Palade, G. E.: Junctional complexes in various epithelia. J. Cell Biol.17, 375–412 (1963).Google Scholar
  4. — —: Cell junctions in amphibian skin. J. Cell Biol.26, 263–291 (1965).Google Scholar
  5. Ilberg, C., v.: Elektronmikroskopische Untersuchung über Diffusion und Resorption von Thoriumdioxyd an der Meerschweinchenschnecke. Arch. Ohr.-, Nas.-, u. Kehlk.-Heilk.190, 415–425 (1968).Google Scholar
  6. Karnovsky, M. J.: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell Biol.35, 213–236 (1967).Google Scholar
  7. Rauch, S.: Biochemie des Hörorganes. Stuttgart: Georg Thieme Verlag 1964.Google Scholar
  8. Spoendlin, H.: Vascular stria. In: Submicroscopic structure of the inner ear, p. 131–149 (S. Iurato, ed.). Oxford: Pergamon Press 1967.Google Scholar
  9. —, Balogh, K.: Histochemical localization of dehydrogenases in the cochlea of living animals. Laryngoscope (St. Louis)73, 1161–1183 (1963).Google Scholar
  10. Vegge, T., Winther, F. Ø., Olsen, B. R.: Horseradish peroxidase in plasma studied by gel filtration. Histochemie (in press.)Google Scholar
  11. Winther, F. Ø.: The permeability of guinea pig cochlear capillaries to horseradish peroxidase. Z. Zellforsch.114, 193–202 (1971).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Finn Ø. Winther
    • 1
    • 2
  1. 1.Anatomical InstituteUniversity of OsloOsloNorway
  2. 2.The Ear, Nose and Throat DepartmentRikshospitaletOsloNorway

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