Archives of Dermatological Research

, Volume 255, Issue 2, pp 139–148 | Cite as

Freeze-etching observations of herpes simplex virus

  • Teruhiko Hasegawa
  • Seiichiro Hata
Article

Summary

Herpes simplex virus isolated from the cheek lesions of a patient was propagated on FL cells. Pellets of infected cells were cryoprotected with glycerol, and processed for freeze-etching. Viral nucleocapsids were observed in the nucleus of FL cells. The cores were observed in three forms. Capsomeres were clearly shown. Release of virus particles from the nucleus and the plasmalemma was more clearly observed. Myeloid figures and lipid droplets were also found. The significance of these findings is discussed.

Keywords

Public Health Lipid Glycerin Infected Cell Herpes Simplex 

Zusammenfassung

Herpes Simplex Virus wurde von Wangenherden eines Patienten auf FL-Zellen gezüchtet, die mit Glycerin kältegeschützt und dem Gefrierätzungsverfahren unterworfen wurden.

Virale Nucleocapside wurden im Kern der FL-Zellen beobachtet. Es wurden drei verschiedene Strukturen erkannt. Die Darstellung von Capsomeren war deutlich. Die Entwicklung von Viruspartikeln vom Kern und dem Plasmalemma wurde deutlich beobachtet. Myeloide Körper und Fetttropfen wurden auch gefunden. Die Bedeutung dieser Ergebnisse wird diskutiert.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Teruhiko Hasegawa
    • 1
  • Seiichiro Hata
    • 2
  1. 1.KyotoJapan
  2. 2.Department of DermatologyOsaka University, School of MedicineOsakaJapan

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