Summary
Herpes simplex virus isolated from the cheek lesions of a patient was propagated on FL cells. Pellets of infected cells were cryoprotected with glycerol, and processed for freeze-etching. Viral nucleocapsids were observed in the nucleus of FL cells. The cores were observed in three forms. Capsomeres were clearly shown. Release of virus particles from the nucleus and the plasmalemma was more clearly observed. Myeloid figures and lipid droplets were also found. The significance of these findings is discussed.
Zusammenfassung
Herpes Simplex Virus wurde von Wangenherden eines Patienten auf FL-Zellen gezüchtet, die mit Glycerin kältegeschützt und dem Gefrierätzungsverfahren unterworfen wurden.
Virale Nucleocapside wurden im Kern der FL-Zellen beobachtet. Es wurden drei verschiedene Strukturen erkannt. Die Darstellung von Capsomeren war deutlich. Die Entwicklung von Viruspartikeln vom Kern und dem Plasmalemma wurde deutlich beobachtet. Myeloide Körper und Fetttropfen wurden auch gefunden. Die Bedeutung dieser Ergebnisse wird diskutiert.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bererhi, A., Malkani, K.: Cryodecapage de Cellules en Culture Infectees par des Virus. microscopie électronique Vol. 1, 457–458, Société Française de Microscopie électronique Paris 1970
Hasegawa, T.: Electron microscopic observations of Hela cells infected with herpes simplex virus. Acta derm. (Kyoto)58, 109–131 (1963)
Hasegawa, T., Uyeda, K.: Ultrastructure of xanthelasma palpebrarum as revealed by freeze etching technique. Arch. Derm. Forsch.248, 347–354 (1974)
Hasegawa, T., Takeuchi, M., Imamura, S.: Ultrastructure of molluscum contagiosum virus. J. invest. Derm.63, 331–333 (1975)
Hasegawa, T., Hata, S.: Freeze-etching observations of herpes simplex virus in the nucleus of FL cell. J. Electron Microscopy24, 43–44 (1975)
Kitano, Y., Hata, S.: The human epidermal cells infected with herpes simplex virus in vitro. Arch. Derm. Forsch.245, 203–210 (1972)
Moor, H.: Etching and related problems. In: Benedetti, E. L., Favard, P. (Edit.), Freeze-etching techniques and applications. p. 21–26. Société Française de Microscopie Électronique, Paris 1973
Nasemann, Th.: Neuere Entwicklungen dermatologischer Virusforschung. XIII. Congr. Inern. Dermatol., München 1967 Bd. I, S. 301–306. Berlin-Heidelberg-New York: Springer 1968
Nermut, M. V.: Freeze-drying and freeze-etching of viruses. In: Benedetti, E. L., Favard, P. (Edit.), Freeze-etching techniques and applications. p. 135–150. Société Française de Microscopie Électronique, Paris 1973
Nii, S.: Electron microscopic observations on FL cells infected with herpes simplex virus. 1. viral forms. Biken's J.14, 177–190 (1971)
Olive, J.: Cryo-ultramicroscopy and freeze-etching of lipid-water phases. In: Benedetti, E. L., Favard, P. (Edit.), Freeze-etching techniques and applications. p. 187 to 198. Société Française de Microscopie Électronique, Paris 1973
Roizman, B., Spear, P. G.: Herpesviruses. In: Daltan, A. J., Hagenau, F. (Edit.), Ultrastructure of animal viruses and bacteriophages: An atlas P. 83–107. New York-London: Academic Press 1973
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hasegawa, T., Hata, S. Freeze-etching observations of herpes simplex virus. Arch. Derm. Res. 255, 139–148 (1976). https://doi.org/10.1007/BF00558521
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00558521