Archives of Dermatological Research

, Volume 253, Issue 1, pp 43–51 | Cite as

Studies on the role of C-type viruses in the development of epithelial tumors induced with DMBA

  • Wojciech Maciejewski
  • Jan Dąbrowski
  • Stefania Jabłńska
Article

Summary

Epithelial tumors were induced using 0.5% solution of DMBA in two strains of mice—one infected with leukoviruses (Swiss mice), and the other free of these viruses (hairless mice). Tumors from 15 mice of each strain were examined light- and electron-microscopically. Depending on the period of administration of the carcinogen, benign growths of the type of papilloma or keratoacanthoma were obtained, or malignant tumors. In the tumors in Swiss mice electron microscopy revealed a distinct increase in the numbers of viruses in comparison with surrounding skin and intact skin of healthy mice of this strain. In spite of certain histologic differences between tumors produced in Swiss and hairless mice, the results argue against a role of leukoviruses in the pathogenesis of experimentally induced epithelial tumors in mice.

Keywords

Papilloma Epithelial Tumor DMBA Swiss Mouse Hairless Mouse 

Zusammenfassung

Epitheliale Tumoren wurden mittels einer 0,5%igen DMBA-Lösung bei 2 Mäusestämmen — einer war infiziert mit Leukoviren (weiße Mäuse vom Swiss-Stamm), der zweite war virusfrei (haarlose Mäuse) — induziert.

Die Tumoren wurden bei 15 Mäusen beider Stämme im Licht- und Elektronenmikroskop untersucht. Abhängig von der Anwendungsperiode des Carcinogens erzielte man benigne Tumoren vom Papillom- und Keratoacanthomtyp oder maligne Tumoren.

Bei den in virusfreien Mäusen erzielten Tumoren zeigten elektronenmikroskopische Untersuchungen ein stärkeres Wachstum der Viren im Verhältnis zur unveränderten Haut in der Tumorumgebung und der gesunden Kontrollmäuse desselben Stammes.

Ungeachtet gewisser histologischer Unterschiede zwischen den Tumoren, induziert bei Mäusen des mit Viren infizierten Stammes und bei haarlosen Mäusen, sprechen die Untersuchungen gegen die Rolle von Leukoviren bei der Pathogenese von experimentellen epithelialen Tumoren bei Mäusen.

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References

  1. 1.
    Arrundell, F. D., Karasch, M. A., Gates, A. H.: 7,12-Dimethylbenzathracene tumor induction in mutant (hairless, asebic and hairless-asebic) mice. J. invest. Derm.52, 119 (1969)Google Scholar
  2. 2.
    Brooke, H. C.: Hairless mice. J. Hered.17, 173 (1926)Google Scholar
  3. 3.
    Feldman, D. G., Gross, L.: Electron microscopic study of the distribution of the mouse leukemia virus Gross in organs of mice and rats with virus-induced leukemia. Cancer Res.26, 412 (1966)Google Scholar
  4. 4.
    Gross, L.: Pathogenic properties and vertical transmission of the mouse leukemia agent. Proc. Soc. exp. Biol. (N.Y.)78, 342 (1951)Google Scholar
  5. 5.
    Hartley, J. W., Rowe, W. P., Capps, W. I., Huchner, R.: Isolation of naturally occurring viruses of the murine leukemia virus group in tissue culture. J. Virol.3, 126 (1969)Google Scholar
  6. 6.
    Irino, S., Ota, Z., Sezaki, T., Suzaki, M., Hirahi, K.: Cellfree transmission of 20-Methyl-cholanthrene-induced RF mouse leukemia and electron microscopic demonstration of virus particles in its leukemic tissue. Gann54, 225 (1963)Google Scholar
  7. 7.
    Kajima, M., Pollard, M.: Virus-like particles in 3-Methylcholanthrene-induced primary and transplanted sarcomas of germ-free rodents. Cancer Res.27, 980 (1967)Google Scholar
  8. 8.
    Liebelt, R. A., Suzuki, S., Liebelt, A. G., Lane, M.: Virus-like particles in chemically induced sarcomas in high- and low-leukemia strains of mice. Cancer Res.30, 2438 (1970)Google Scholar
  9. 9.
    Luft, J. M.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 400 (1961)Google Scholar
  10. 10.
    Melnick, J. L.: Papova virus group. Science135, 1128 (1962)Google Scholar
  11. 11.
    Nowinski, R. G., Old, L. J., Sarker, N. H., Moore, D. H.: Common properties of the oncogenic RNA viruses (Oncornaviruses). Virology42, 1152 (1970)Google Scholar
  12. 12.
    Rappaport, H., Baroni, C.: A study of the pathogenesis of malignant lymphoma induced in the Swiss mouse with 7:12 dimethylbenzanthracene injected at birth. Cancer Res.22, 1067 (1962)Google Scholar
  13. 13.
    Reynolds, E. S.: The use of lead citrate at hight pH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208 (1963)Google Scholar
  14. 14.
    Svoboda, D.: Ultrastructure of pulmonary adenomas in mice. Cancer Res.22, 1197 (1962)Google Scholar
  15. 15.
    Toth, B.: Development of malignant lymphomas by cell-free filtrates prepared from a chemically-induced mouse lymphoma. Proc. Soc. exp. Biol. (N.Y.)112, 873 (1963)Google Scholar
  16. 16.
    Zilber, L., Postnikowa, Z.: Induction of a leukemogenic agent by a chemical carcinogen in inbred mice. Nat. Cancer Inst. Monogr.22, 397 (1966)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Wojciech Maciejewski
    • 1
  • Jan Dąbrowski
    • 1
  • Stefania Jabłńska
    • 1
  1. 1.Department of DermatologyWarsaw Medical AcademyPoland

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