Summary
Adult female Wistar rats were fed with a diet containing 0.05% HCB. About the 55th day of the experiments an increase of porphyrin and its precursors (ALA and PBG) in the urine of the rats can be measured. In contrast the induction of the O-dealcylation reaction of 7-ethoxycoumarin could be already measured 2 days after starting with the HCB feeding. The inhibition of the enzymatic reaction by metyrapone, naphthoflavone, tetrahydrofurane or CO/O2 presents neither the typical pattern of the phenobarbital type nor the typical pattern of the benzpyrene type of cytochrome P-450. When the animals became porphyric (about the 55th day of the HCB exposure) no qualitative changes in the cytochrome P-450 pattern could be demonstrated. The relationship between the enzyme induction in the liver and the porphyria following the HCB application is still remaining unclear.
Zusammenfassung
Weibliche Wistar-Ratten wurden kontinuierlich mit 0,05% HCB-Futter ernährt. Zwischen dem 54.–57. Tag der HCB-Exposition kommt es bei den Versuchstieren zu einem steilen Anstieg der Porphyrin-Ausscheidung und der Vorstufen (ALA und PBG) im Urin. Im Gegensatz dazu läßt sich bereits am 2. Tag der HCB-Applikation eine Steigerung der O-Desalkylierungs-Aktivität nachweisen. Bei der Anwendung der verschiedenen Inhibitoren: Metyrapon, Naphthoflavon, Tetrahydrofuran und CO/O2 läßt sich das HCB-induzierte Cytochrom weder dem Phenobarbital- noch dem Benzpyren-Typ des Cytochrom P-450 eindeutig zuordnen.
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Goerz, G., Krieg, T., Bolsen, K. et al. Langzeit-exposition von ratten mit hexachlorbenzol (HCB): Einfluß auf die porphyrin-ausscheidung im urin und auf das cytochrom P-450 in der leber. Arch. Derm. Res. 259, 199–206 (1977). https://doi.org/10.1007/BF00557961
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DOI: https://doi.org/10.1007/BF00557961