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The epidermal cell migration promoting activity of serum in guinea pig skin in vitro

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Summary

  1. 1.

    Explants of skin taken from albino guinea pigs have been cultured in the presence of various sera and serum substitutes in an attempt to define the “promoting activity” of serum for epidermal migrationin vitro.

  2. 2.

    On analysis autologous serum was found to be more effective in promoting migration than the non-autologous sera types investigated.

  3. 3.

    The serum factor(s) thought to be associated with migration are not destroyed by heating and are lost in dialysis. It seems that they are not complex high molecular weight substances.

  4. 4.

    Investigations with serum substitutes suggest that epidermal cell migrationin vitro is not dependant on the tonicity of the medium.

Zusammenfassung

Hautstückchen von Albinomeerschweinchen wurden als Explantatkulturen in Gegenwart verschiedener Seren, Serumsubstitutein vitro gezüchtet, um die stimulierende Serumaktivität auf epidermale Zellmigration zu untersuchen. Es zeigte sich, daß autologes Serum im Vergleich zu nicht-autologen Seren verschiedener Herkunft eine stärker stimulierende Wirkung auf die Zellmigration hat.

Die Serumbestandteile mit migrationsfördernder Wirkung werden nicht durch Hitze inaktiviert, gehen jedoch durch Dialyse verloren. Es handelt sich wahrscheinlich nicht um hochmolekulare Substanzen.

Die Verwendung von Serumsubstituten deutet darauf hin, daß die epidermale Zellmigrationin vitro nicht von der Tonizität des Mediums abhängig ist.

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Authors and Affiliations

  1. Department of Experimental Pathology, The Institute of Dermatology, London, England

    Valerie-Anne Coombs, B. K. Nissen & R. Marks

  2. St. John's Hospital for Diseases of the Skin, London, England

    Valerie-Anne Coombs, B. K. Nissen & R. Marks

Authors
  1. Valerie-Anne Coombs
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  2. B. K. Nissen
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  3. R. Marks
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In receipt of a grant from the M.R.C.

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Coombs, VA., Nissen, B.K. & Marks, R. The epidermal cell migration promoting activity of serum in guinea pig skin in vitro. Arch. Derm. Res. 249, 367–372 (1974). https://doi.org/10.1007/BF00557897

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  • DOI: https://doi.org/10.1007/BF00557897

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