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Genetics of esterases inDrosophila. IX. Characterization of the JH-esterase inD. virilis

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Abstract

The kinetic characteristics of the main isozymes ofDrosophila virilis esterase were studied andK m values of esterase-2, -4, and -6 and p-esterase for α- and β-naphthyl acetate were obtained. Juvenile hormone (JH) was shown to inhibit the p-esterase activity when in competition with β-naphthyl acetate and the general esterase inhibitor, diisopropylphosphofluoridate (DFP), was shown to inhibit all the components of theD. virilis esterase patterns except p-esterase. While studying the changes of p-esterase activity inD. virilis ontogenesis, the increase in p-esterase activity in the wandering larvae, prepupae, and early pupae was found to correlate with a decrease in JH titer at these stages. The decrease in JH level in a temperature-sensitive lethal mutant larvae ofD. virilis at high temperatures was shown to correlate with increased p-esterase activity. These results confirm that p-esterease ofD. virilis is JH-esterase.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Department, USSR Academy of Sciences, Novosibirsk, USSR

    I. Yu. Rauschenbach & N. S. Lukashina

  2. Institute of Bioorganic Chemistry, Siberian Department, USSR Academy of Sciences, Novosibirsk, USSR

    V. G. Budker

  3. Institute of Developmental Biology, USSR Academy of Sciences, Moscow, USSR

    L. I. Korochkin

Authors
  1. I. Yu. Rauschenbach
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  2. N. S. Lukashina
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  3. V. G. Budker
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  4. L. I. Korochkin
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Rauschenbach, I.Y., Lukashina, N.S., Budker, V.G. et al. Genetics of esterases inDrosophila. IX. Characterization of the JH-esterase inD. virilis . Biochem Genet 25, 687–704 (1987). https://doi.org/10.1007/BF00556212

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  • DOI: https://doi.org/10.1007/BF00556212

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