Abstract
The chromatin of a mouse that is trisomic for part of chromosome 7 was investigated. Chromatin from trisomic tissue has a smaller average nucleosome DNA repeat length than chromatin from tissue taken from normal diploid littermates. DNA of the nucleosome cores is the same size in both normal and trisomic tissues. Not all of the nucleosome monomers have different repeat lengths. Normal and trisomic mouse kidney cells in tissue culture maintained their nucleosome repeat-length differences.
Similar content being viewed by others
References
Burgoyne, L. A., Wagar, M. A., and Atkinson, M. R. (1970). Calcium-dependent priming of DNA synthesis in isolated rat liver nuclei.Biochem. Biophys. Res. Comm. 39254.
Eicher, E. M., and Coleman, D. L. (1977). Influence of gene duplication and X inactivation on mouse mitochondrial malic enzyme activity and electrophonetic patterns.Genetics 85647.
Fedorova, K. N. (1977). The structure of human cell interphase chromatin in the mosaic form of Down's disease.J. Ment. Defic. Res. 2185.
Fedorova, K. N., and Yudina, I. E. (1981). Melting profiles of lymphocyte chromatin in Down's syndrome detected by optical density technique.Biull. Eksp. Biol. Med. 91208.
Fedorova, K. N., Spitkovsky, D. M., and Seregina, V. N. (1977). The structure of interphasic chromatin in the patients with Down's syndrome and in their mothers.Biull. Eksp. Biol. Med. 83353.
Fedorova, K. N., Sachepilou, V. N., and Parfenova, I. V. (1980). Changes in the fibroblast chromatin structure in a patient with Down's disease and in his mother.Biull. Eksp. Biol. Med. 89603.
Helton, D. R., and Lucot, J. B. (1986). Behavioral and developmental changes in murine T(X, 7) Ct (submitted for publication).
Inshakova, V. M., Fedorova, K. N., and Spitkovsky, D. M. (1979). Blood serum as a factor of chromatin condensation on trisomy-21 (Down's syndrome).Biull. Eksp. Biol. Med. 88460.
Maniatis, E. G., Fritsch, E., and Sambrook, J. (1982).Molecular Cloning. A Laboratory Manual Cold Spring Harbor, Laboratory, Cold Spring Harbor, N.Y., pp. 454.
Sanders, M. M. (1978). Fractionation of nucleosomes by salt clution from micrococcal nuclease-digested nuclei.J. Cell Biol. 7997.
Thomas, J. O., and Thompson, R. J. (1977). Variation in chromatin structure in two cell types from the same tissue: A short DNA repeat length in cerebral cortex neurons.Cell 10633.
Todd, R. D., and Garrard, W. T. (1979). Overall pathway of mononucleosome production.J. Biol. Chem. 2543074.
Weintraub, H., and Groudine, M. (1976). Chromosomal subunits in active genes have an altered conformation.Science 193848.
Author information
Authors and Affiliations
Additional information
Partially supported by institutional Biomedical Seed Grant 660228.
Rights and permissions
About this article
Cite this article
Rake, A.V., Edwards, R.H. Altered chromatin structure associated with a partial trisomy of chromosome 7 in the mouse. Biochem Genet 25, 671–686 (1987). https://doi.org/10.1007/BF00556211
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00556211