Biochemical Genetics

, Volume 26, Issue 1–2, pp 143–151 | Cite as

Betaine aldehyde dehydrogenase polymorphism in spinach: Genetic and biochemical characterization

  • Elizabeth A. Weretilnyk
  • Andrew D. Hanson
Article

Abstract

Spinach (Spinacia oleracea L.) has a major chloroplastic isozyme of betaine aldehyde dehydrogenase (BADH) and a minor cytosolic one. Among a diverse collection of spinach accessions, three electrophoretic banding patterns of chloroplastic BADH were found: two were single banded and one was triple banded. Genetic analysis of these patterns indicated that chloroplastic BADH is encoded by a single, nuclear gene with two alleles, designated slow (S) and fast (F), and that products of these alleles can hybridize to form either homodimers or a heterodimer. The S allele was by far the most common among the accessions examined. Native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the SS and FF homodimers differ in charge but not molecular weight.

Key words

Spinacia oleracea betaine aldehyde dehydrogenase isozymes polymorphism chloroplasts salt stress 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Elizabeth A. Weretilnyk
    • 1
  • Andrew D. Hanson
    • 1
  1. 1.MSU-DOE Plant Research LaboratoryMichigan State UniversityEast Lansing

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