Abstract
Analyses of wheat/rye addition lines by Southern blotting confirmed the presence of sequences related to theSec 1, Sec 2, andSec 3 loci on chromosomes 1R and 2R. Comparison of the 1R and 2R addition lines allowed the identification of γ-secalin genes atSec 1 andSec 2, respectively, while ω-secalin and γ-secalin genes atSec 1 were discriminated by comparative hybridization with three probes: ω-secalin, total γ-secalin, and 3′ γ-secalin. The high molecular weight (HMW) secalin genes atSec 3 were identified using a homologous HMW subunit probe from wheat. Gene copy numbers were estimated as about 40–60 for ω-secalins, 5–10 for γ-secalins, and 2 for HMW secalins. Comparison of individual plants of cv. Gazelle showed a high degree of polymorphism, particularly for sequences related to ω-secalins and HMW secalins.
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Hull, G., Sabelli, P.A. & Shewry, P.R. Restriction fragment analysis of the secalin loci of rye. Biochem Genet 30, 85–97 (1992). https://doi.org/10.1007/BF00554430
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DOI: https://doi.org/10.1007/BF00554430