Biochemical Genetics

, Volume 25, Issue 7–8, pp 513–526 | Cite as

Molecular characterization of meiotic recombination within the major histocompatibility complex of the mouse: Mapping of crossover sites within theI region

  • Howard C. Passmore
  • Joan A. Kobori
  • Edmund J. Zimmerer
  • Dominic G. Spinella
  • Leroy Hood
Article

Abstract

The molecular analysis of crossing-over within the mouse major histocompatibility complex provides a useful approach for the study of the structural characteristics of meiotic recombination. In this study five intra-I-region recombinants, each derived fromIk/Ib heterozygotes, were characterized for restriction-fragment length polymorphisms (RFLPs) characteristic of theI region of the two parental strains. Southern blot analysis of intra-I recombinant strains A.TBR2, A.TBR3, A.TBR5, A.TBR13, and A.TBR17 using sixI-region DNA probes revealed that the point of crossing-over in all five recombinants occurred within a 6.2-kbKpnI-EcoRI segment located within theEβ gene. The segments of DNA containing the crossover point from each of the recombinant chromosomes were cloned by screening partial genomic libraries constructed in λgt7 bacteriophage. Construction of partial restriction maps of the cloned segments from the parental and recombinant chromosomes permitted the boundaries of the area containing the crossover site to be narrowed to a 4.0-kb segment located almost entirely within an intron of theEβ gene. The recognition that the points of crossing-over in all five recombinants studied are clustered in a relatively small area of theI region provides further evidence for a hot spot of recombination associated with theEß gene.

Key words

crossing-over recombination restriction-fragment length polymorphisms (RFLPs) major histocompatibility complex (MHC) 

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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Howard C. Passmore
    • 1
    • 2
  • Joan A. Kobori
    • 1
  • Edmund J. Zimmerer
    • 2
  • Dominic G. Spinella
    • 2
  • Leroy Hood
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadena
  2. 2.Department of Biological SciencesRutgers UniversityPiscataway

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