Biochemical Genetics

, Volume 27, Issue 5–6, pp 303–312 | Cite as

Biochemical correlates of genetic variation in marine lower invertebrates

  • A. M. Sole-Cava
  • J. P. Thorpe
Article

Abstract

In this paper extensive data on enzyme variation in 23 species of coelenterates and sponges were used to investigate the possible correlation of levels of genetic variation with various parameters of enzyme molecular structure and function. The data provide an opportunity not only to look for such correlations for the first time in lower invertebrates, but also to study organisms with far higher average levels of genetic variability than those used in any previous work. A clear inverse relationship was found between enzyme subunit number and levels of polymorphism, with monomers being more variable than dimers or tetramers. No significant difference in polymorphism could be found in enzymes of the functional groups I and II of Gillespie and Langley (1974). Regulatory enzymes appeared to be significantly more polymorphic than nonregulatory enzymes, but a significant relationship was observed between regulatory power and subunit structure which could bias this result. The results suggest that both neutralist and selectionist ideas may have a useful role to play in the understanding of the factors which can influence or limit levels of genetic variation.

Key words

heterozygosity neutralism selectionism Porifera Coelenterata enzyme structure enzyme function 

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References

  1. Ayre, D. J. (1984). The effects of sexual and asexual reproduction on geographic variation in the sea anemoneActinia tenebrosa.Oecologia 62222.Google Scholar
  2. Bergquist, P. R. (1978).Sponges Hutchinson, London.Google Scholar
  3. Brewer, G. J. (1970).An Introduction to Isozyme Techniques. Academic Press, New York.Google Scholar
  4. Dickenson, R. E. (1971). The structure of cytochrome c and the rates of molecular evolution.J. Mol. Evol. 126.Google Scholar
  5. Dixon, M., and Webb, E. C. (1979).Enzymes 3rd ed., Longman, London.Google Scholar
  6. Fincham, J. R. S. (1972). Heterozygous advantage as a likely general basis for enzyme polymorphisms.Heredity 28387.Google Scholar
  7. Gillespie, J. H., and Kojima, K. (1968). The degree of polymorphism in enzymes involved in energy production compared to that in non-specific enzymes in twoDrosophila ananassae populations.Genetics 61837.Google Scholar
  8. Gillespie, J. H., and Langley, C. H. (1974). A general model to account for enzyme variation in natural populations.Genetics 76837.Google Scholar
  9. Gojobori, T. (1982). Means and variances of heterozygosity and protein function. In Kimura, M. (ed.),Molecular Evolution, Protein Polymorphism and the Neutral Theory Japan Scientific Society Press, Tokyo, Springer-Verlag, Berlin.Google Scholar
  10. Harris, H., and Hopkinson, D. A. (1978).Handbook of Enzyme Electrophoresis in Human Genetics North-Holland, Amsterdam.Google Scholar
  11. Harris, H., Hopkinson, D. A., and Edwards, Y. H. (1977). Polymorphism and the subunit structure of enzymes: A contribution to the neutralist-selectionist controversy.Proc. Natl. Acad. Sci. USA 74698.Google Scholar
  12. Hochachka, P. W., and Somero, G. N. (1984).Biochemical Adaptation Princeton University Press, Princeton, N.J.Google Scholar
  13. Hopkinson, D. A., Edwards, Y. H., and Harris, H. (1976). The distribution of subunit numbers and subunit sizes of enzymes: A study of the products of 100 human loci.Ann. Hum. Genet. 39383.Google Scholar
  14. Johnson, G. B. (1971). Metabolic implications of polymorphism as an adaptive strategy.Nature 232347.Google Scholar
  15. Johnson, G. B. (1973). Importance of substrate variability to enzyme polymorphism.Nature New Biol. 243151.Google Scholar
  16. Johnson, G. B. (1974). Enzyme polymorphism and metabolism.Science 18428.Google Scholar
  17. Johnson, G. B. (1976). Genetic polymorphism and enzyme function. In Ayala, F. J. (ed.),Molecular Evolution Sinauer Associates, Sunderland, Mass.Google Scholar
  18. Kimura, M. (1968). Evolutionary rate at the molecular level.Nature 217624.Google Scholar
  19. Kimura, M. (1983).The Neutral Theory of Molecular Evolution Cambridge University Press, London.Google Scholar
  20. Kimura, M., and Ohta, T. (1974). On some principles governing molecular evolution.Proc. Natl. Acad. Sci. USA 712848.Google Scholar
  21. Koehn, R. K., and Eanes, W. F. (1977). Subunit size and genetic variation of enzymes in natural populations ofDrosophila.Theor. Pop. Biol. 11330.Google Scholar
  22. Koehn, R. K., and Eanes, W. F. (1978). Molecular structure and protein within and among populations.Evol. Biol. 1139.Google Scholar
  23. Kojima, K., Gillespie, J., and Tobari, Y. N. (1970). A profile ofDrosophila species enzymes assayed by electrophoresis. I. Numbers of alleles, heterozygosities and linkage disequilibrium in glucose metabolizing systems and some other enzymes.Biochem. Genet. 4627.Google Scholar
  24. Lehninger, A. L. (1975).Biochemistry Worth, New York.Google Scholar
  25. Nei, M., Fuerst, P. A., and Chakraborty, R. (1978). Subunit molecular weight and genetic variability of proteins in natural populations.Proc. Natl. Acad. Sci. USA 753359.Google Scholar
  26. Nelson, K., and Hedgecock, D. (1980). Enzyme polymorphism and adaptive strategy in the decapod Crustacea.Am. Nat. 116238.Google Scholar
  27. Nevo, E. (1978). Genetic variation in natural populations: patterns and theory.Theor. Pop. Biol. 13121.Google Scholar
  28. Nevo, E. (1983). Adaptive significance of protein variation. In Oxford, G. S., and Rollinson, D. (eds.),Protein Polymorphism: Adaptive and Taxonomic Significance Academic Press, London.Google Scholar
  29. Powell, J. R. (1975). Protein variation in natural populations of animals.Evol. Biol. 879.Google Scholar
  30. Quicke, D. L. J., and Brace R. C. (1983). Phenotypic and genotypic spacing within an aggregation of the sea anemone,Actinia equina.J. Mar. Biol. Assoc. U.K. 63493.Google Scholar
  31. Ruth, R. C., and Wold, F. (1976). The subunit structure of glycolytic enzymes.Comp. Biochem. Physiol. 54B1.Google Scholar
  32. Selander, R. K. (1976). Genetic variation in natural populations. In Ayala, F. J. (ed.),Molecular Evolution Sinauer Associates, Sunderland, Mass.Google Scholar
  33. Smith, P. J., and Fujio, Y. (1982). Genetic variation in marine teleosts: High variability in habitat specialists and low variability in habitat generalists.Mar. Biol. 697.Google Scholar
  34. Ward, R. D. (1977). Relationship between enzyme heterozygosity and quaternary structure.Biochem. Genet. 15123.Google Scholar
  35. Ward, R. D. (1978). Subunit size of enzymes and genetic heterozygosity in vertebrates.Biochem. Genet. 16779.Google Scholar
  36. Ward, R. D., and Skibinski, D. O. F. (1988). Evidence that mitochondrial enzymes are genetically less variable than cytoplasmic enzymes.Genet. Res. Cambr. 51121.Google Scholar
  37. Zouros, E. (1975). Electrophoretic variation in allozymes related to function or structure?Nature 254446.Google Scholar
  38. Zouros, E. (1976). Hybrid molecules and the superiority of the heterozygote.Nature 262227.Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • A. M. Sole-Cava
    • 1
  • J. P. Thorpe
    • 1
  1. 1.Department of Marine BiologyThe University of Liverpool, Port Erin Marine Laboratory, Port Erin, Isle of ManUK

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