Biochemical Genetics

, Volume 29, Issue 7–8, pp 337–344 | Cite as

Genetics and polymorphism of a duplicated malate dehydrogenase (MDH) locus in the grasshopperOxya japonica japonica (Orthoptera:Acrididae:Oxyinae)

  • K. L. Chan
  • Y. Yushayati
  • P. Guganeswaran


A biochemical genetic study of the enzyme malate dehydrogenase (MDH) was conducted in the grasshopperOxya j. japonica. Analysis of MDH electrophoretic variation in this species of grasshopper shows that one of the two autosomal loci for MDH in grasshoppers, the Mdh-2 locus, controlling the anodal set of MDH isozymes, is duplicated. Results of breeding studies confirm this and the observed polymorphism at theMdh-2 locus in the two populations ofOxya j. japonica studied can be attributed to three forms of linked alleles at the duplicated locus in equilibrium in both populations. In this respect, all individuals of this species possess heterozygous allelic combinations at the duplicatedMdh-2 locus, which may account for the spread of the duplicated locus in the populations of this species of grasshopper.

Key words

grasshopper Oxya japonica japonica malate dehydrogenase duplicated gene polymorphism 


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  1. Daly, J. C., Wilkinson, P., and Shaw, D. D. (1981). Reproductive isolation in relation to allozymic and chromosomal differentiation in the grasshopperCaledia captiva. Evolution351164.Google Scholar
  2. Ernst, S. G., Keathley, D. E., and Hanover, J. W. (1987). Inheritance of isozymes in seed and bud tissue of blue and Engelmann spruce.Genome 29239.Google Scholar
  3. Gill, P. (1981a). Heterozygosity estimates in the grasshopperChorthippus brunneus (Thunberg).Heredity 46269.Google Scholar
  4. Gill, P. (1981b). Enzyme variation in the grasshopperChorthippus brunneus (Thunberg).Biol. J. Linn. Soc. 15247.Google Scholar
  5. Gill, P. (1981c). Allozyme variation in sympatric populations of British grasshoppers—evidence of natural selection.Biol. J. Linn. Soc. 1683.Google Scholar
  6. Gottlieb, L. D. (1974). Gene duplication and fixed heterozygosity for alcohol dehydrogenase in the diploid plantClarkia franciscana.Proc. Natl. Acad. Sci. USA 711819.Google Scholar
  7. Gottlieb, L. D. (1977). Evidence for duplication and divergence of the structural gene for phosphogluco isomerase in diploid species ofClarkia.Genetics 86289.Google Scholar
  8. Halliday, R. B., Barton, N. H., and Hewitt, G. H. (1983). Electrophoretic analysis of a chromosomal hybrid zone in the grasshopperPodisma pedestris.Biol. J. Linn. Soc. 1951.Google Scholar
  9. Hollis, D. (1971). A preliminary revision of the genusOxya Audimet-Serville (Orthoptera: Acridoidae).Bull. Br. Mus. Nat. His. 26269.Google Scholar
  10. Honeycutt, R. L. (1989). Electrophoretic variation in the parthenogenetic grasshopperWarramaba virgo and its sexual relatives.Evolution 431027.Google Scholar
  11. Jeffreys, A. J. (1982). Evolution of hemoglobin genes. In Dover, G. A., and Flavell, R. B. (eds.),Genome Evolution Academic Press, New York.Google Scholar
  12. McMillin, D. E., and Scandalios, J. G. (1980). Duplicated cytosolic malate dehydrogenase genes inZea mays.Proc. Natl. Acad. Sci. USA 774866.Google Scholar
  13. Morgan, C., Wilkinson, P., and Shaw, D. D. (1980). Allozyme variation across a narrow hybrid zone in the grasshopper,Caledia captiva.Heredity 4469.Google Scholar
  14. Smithies, O., Connell, G. E., and Dixon, G. H. (1962). Chromosomal rearrangements and evolution of haptoglobin genes.Nature 196232.Google Scholar
  15. Yong, H. S., and Mak, J. W. (1984). Glucose phosphate isomerase heterophenotypes in the human filarial parasiteBrugia malayi from Peninsula Malaysia.Experientia 40833.Google Scholar
  16. Yong, H. S., Mak, C., Chan, K. L., and Dhaliwal, S. S. (1982). Inheritance of isocitrate dehydrogenase in the cultivated soybean.Malay. Nat. J. 35225.Google Scholar
  17. Yong, H. S., Mak, J. W., and Lim, P. K. C. (1985). Glucose phosphate isomerase polymorphism in subperiodicBrugia malayi (Nematoda:Filarioidea) passaged from an Orange Asli in Peninsula Malaysia.Trop. Biomed. 270.Google Scholar

Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • K. L. Chan
    • 1
  • Y. Yushayati
    • 1
  • P. Guganeswaran
    • 1
  1. 1.Department of Genetics & Cellular BiologyUniversity of MalayaKuala LumpurMalaysia

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