Abstract
In an attempt to elucidate the effect of metallic ions and EDTA on acidic α-d-glucosidase activity, we measured acidic α-d-glucosidase activity from either lymphocyte and muscle tissue homogenates or intact cells after incubation with metallic ions. The results showed that this enzyme activity was strongly inhibited by Ag+, Hg2+, and Fe3+ in either lymphocyte or muscle tissue homogenates. There was no effect of Zn2+, Cu2+, and Cd2+. However, intact cells, either lymphocyte or muscle cells, after incubation with Zn2+ for 1 or 2 hr, showed enhanced enzyme activity and suppression in the other metallic ion groups, especially in Ag+, Hg2+, and Fe3+. Since deficiency of this enzyme can cause type II glycogen storage disese (Pompe's disease), the more we understand the character of this enzyme, the more we can improve our enzymatic therapy.
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This work was supported by Grant NSC75-0412-B075-41 from the National Science Council of the Republic of China.
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Lin, CY., Hwang, B. Zinc can activate cellular acidic α-d-glucosidase activity. Biochem Genet 26, 323–329 (1988). https://doi.org/10.1007/BF00554068
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DOI: https://doi.org/10.1007/BF00554068