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Histochemistry

, Volume 80, Issue 6, pp 563–567 | Cite as

Localization of filipin-sterol complexes in cell membranes of eosinophils

  • P. F. P. Pimenta
  • W. de Souza
Article

Summary

The polyene antibiotic filipin was used as a probe for the detection of cholesterol in the cell membranes of eosinophils isolated from the peritoneal exudate of rats. A homogenous distribution of filipin-sterol complexes was observed, both in thin sections and freeze-fracture replicas throughout the whole plasma membrane but not in the membrane of pynocytic vesicles, Golgi complex, endoplasmic reticulum, mitochondria and the nucleus. Few complexes were seen in freeze-fracture replicas showing the membrane of the specific granules. Treatment of living cells with filipin induced aggregation of filipin-sterol complexes at some points of the plasma membrane.

Keywords

Public Health Cholesterol Cell Membrane Endoplasmic Reticulum Living Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • P. F. P. Pimenta
    • 1
  • W. de Souza
    • 1
  1. 1.Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, and Laboratório de Ultraestrutura CelularUniversidade Federal do Rio de Janeiro, Ilha do Fundão, 21941Rio de JaneiroBrazil

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