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Histochemistry

, Volume 80, Issue 6, pp 547–552 | Cite as

Innermembrane association of three mitochondrial β-oxidation enzymes revealed by immunoelectron microscopic technique

  • S. Yokota
  • T. Hashimoto
Article
  • 19 Downloads

Summary

Ultrastructural localization of three mitochondrial β-oxidation enzymes, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase in rat liver was studied by a post-embedding immunocytochemical technique. Rat liver was fixed by perfusion. Vibratome sections (100 μm thick) were embedded in Lowicryl K4M. Ultrathin sections were separately incubated with antibody to each enzyme, followed by protein A-gold complex. Gold particles representing the antigenic sites for all enzymes examined were confined exclusively to mitochondria of hepatocytes and other sinus-lining cells. Peroxisomes were consistently negative for the immunolabelling. In the mitochondria the gold particles were localized in the matrical side of inner membrane. The control experiments confirmed the specificity of the immunolabelling. The results firstly indicate that the mitochondrial β-oxidation enzymes are present in the matrix of mitochondria and associated with the inner membrane.

Keywords

Public Health Gold Control Experiment Gold Particle Ultrathin Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Yokota
    • 1
  • T. Hashimoto
    • 2
  1. 1.Department of AnatomyYamanashi Medical SchoolYamanashiJapan
  2. 2.Department of BiochemistryShinshu University School of MedicineNaganoJapan

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