Advertisement

Histochemistry

, Volume 80, Issue 6, pp 535–538 | Cite as

Rat extraocular muscle

3. Histochemical variability along the length, of multiply-innervated fibers of the orbital surface layer
  • B. R. Pachter
Article

Summary

Multiply-innervated fibers from rat superior oblique extraocular muscle were followed in sequential serial sections using histochemistry. Sudan black and adenosine-triphosphase (ATPase) histochemical staining reactions were used to identify these fibers in the muscle's global layer and orbital surface layer. Regional differences in ATPase staining occurred along the length of multiply-innervated fibers from the orbital surface layer. In their middle third where these fibers appear ‘morphologically-fast’ and contain endplatelike endings, they were found to exhibit a dual ATPase activity. In their distal third where they appear ‘morphologically-slow’ and contain simple, superficial endings, they had an alkaline-labile and acid-stabile ATPase activity profile. In contrast, the multiply-innervated fibers of the global layer exhibited a homogeneous ATPase activity, i.e., alkaline-labile and acid-stabile pattern. This histochemical homogeneity parallels their uniform morphologically-slow profile. These fibers contain only multiple superficial endings. It would appear that the histochemical and ultrastructural profile of a fiber is dependent upon the type and location of the motor innervation.

Keywords

Public Health Surface Layer ATPase Activity Regional Difference Serial Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Davidowitz J, Pachter BR, Phillips G, Breinin GM (1977) Systematic morphological variability along the length of muscle fibers in extraocular muscles of. rabbit. A study in serial sections. 35th Ann Proc of the Electron Microscopy Soc Amer, pp 566–567Google Scholar
  2. Farrell P, Fedde MR (1969) Uniformity of structural characteristics throughout the length f skeletal muscle fibers. Anat Rec 164:219–230Google Scholar
  3. Guth L, Samaha FJ, Albers RW (1970) The neural regulation of some phenotypic differences between the fiber types of mammalian skeletal muscle. Exp Neurol 26:126–140Google Scholar
  4. Hanson J, Lennerstrand G (1977) Contractile and histochemical properties of the inferior oblique muscle in the rat and in the cat. Acta Ophthalmol 55:88–102Google Scholar
  5. Hess A (1970) Veterbrate slow muscle fibers. Physiol Rev 50:40–62Google Scholar
  6. Kucera J (1982) A study of motor nerve terminals on cat nuclear bag, intrafusal muscle fibers using the ChE staining technique. Anat Rec 202:407–418Google Scholar
  7. Kucera J, Dorovini-Zis K (1979) Types of human intrafusal muscle fibers. Muscle Nerve 2:437–451Google Scholar
  8. Kucera J, Dorovini-Zis K, Engel WK (1978) Histochemistry of rat intrafusal muscle fibers and their motor innervation. J Histochem Cytochem 26:973–988Google Scholar
  9. Lennerstrand G (1975) Motor units in eye muscles. In: Lennerstrand G, Bach-y-Rita P (eds) Basic mechanisms of ocular motility and their clinical implications. Pergamon Press, Oxford, pp 119–143Google Scholar
  10. Lennerstrand G, Nichols KC (1977) Morphology of motor units in cat extraocular muscle. Acta Ophthalmol 55:913–918Google Scholar
  11. Manolides L, Balogannes S (1981) Histological and ultrastructural findings in the vocal muscles of patients suffering from muscular dystrophies. Acta Otorhinolaryngol 230:181–188Google Scholar
  12. Nemeth PM, Pette D, Vrbova G (1980) Malate dehydrogenase homogeneity of single fibers of the motor unit. In: Pette D, (ed) Plasticity of muscle. Walter de Gruyter, Berlin New York, pp 45–57Google Scholar
  13. Pachter BR (1982) Fiber composition of the superior rectus extraocular muscle of the Rhesus macaque. J Morphol 174:237–250Google Scholar
  14. Pachter BR (1983) Rat extraocular muscle. I. Three dimensional cytoarchitecture, component fibre populations and innervation. J Anat 137:143–159Google Scholar
  15. Pachter BR, Colbjornsen C (1983) Rat extraocular muscle. 3. Histochemical fibre types. J Anat 137:161–170Google Scholar
  16. Pachter BR, Davidowitz J, Breinin GM (1976) Light and electron microscopic analysis of mouse extraocular muscle: Morphology, innervation and topographical organization of component fiber populations. Tissue Cell 8:547–560Google Scholar
  17. Schmalbruch H (1967) Fasertypen in der Unterschenkelmuskulatur der Maus. Zellforsch mikrosk Anat 79:64–75Google Scholar
  18. Veggetti A, Mascarello F, Carysene E (1982) A comparative histochemical study of fibre types in middle ear muscles. J Anat 135:333–352Google Scholar
  19. Yellin H (1969) Unique intrafusal and extraocular muscle fibers exhibiting dual actomyosin ATPase activity. Exp Neurol 25:153–163Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • B. R. Pachter
    • 1
  1. 1.Department of Rehabilitation MedicineNew York University Medical CenterNew YorkUSA

Personalised recommendations